Rabbit anti-Human TKT Polyclonal Antibody | anti-TKT antibody

Anti-TKT Antibody, Rabbit Polyclonal

Cat. Num. AAA8119265

• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: TKT; Polyclonal Antibody; Anti-TKT Antibody; Rabbit Polyclonal; TKT Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-HEL-S-48 Antibody; Anti-HEL107 Antibody; Anti-TK Antibody; Anti-TKT1 Antibody; transketolase; anti-TKT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TKT
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid. PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-TKT antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 1-5uL/mg of Lysate
• Immunogen: E Coli-derived Human TKT fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human TKT fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.; This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Western Blot (WB)

(Anti-TKT rabbit polyclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:68 kDa Observed band size:70 kDa)

Immunoprecipitation (IP)

(TKT was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL anti-TKT rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TKT rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 68 kDa Observed band size :68 kDa)

Related Product Information for anti-TKT antibody

Background: Transketolase (TKT) is a thiamine pyrophosphate (vitamin B1)-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway by feeding excess sugar phosphates into the main carbohydrate metabolic pathways to generate biosynthetic reducing capacity in the form of NADPH as a substrate for ROS generation. TKT is a key enzyme of the pentose phosphate pathway (PPP), the Calvin cycle, and the ribulose monophosphate (RuMP) cycle. B. methanolicus MGA3 harbors two genes putatively coding for TKTs; one located on the chromosome (tkt(C)) and one located on the natural occurring plasmid pBM19 (tkt(P)). The metabolic enzyme TKT in the nonoxidative branch of the PPP regulates ribose 5-phosphate (R5P) levels and de novo nucleotide biosynthesis. TKT is required for maintaining cell proliferation in human liver cancer cell lines.

References

Yang H, et al. (2016) Microrna-497 regulates cisplatin chemosensitivity of cervical cancer by targeting transketolase. Am J Cancer Res 6 (11): 2690-2699.Riyapa D, et al. (2019) Transketolase and vitamin b1 influence on ros-dependent neutrophil extracellular traps (nets) formation. PLoS One 14 (8): e0221016.Markert B, et al. (2014) Characterization of two transketolases encoded on the chromosome and the plasmid pbm19 of the facultative ribulose monophosphate cycle methylotroph bacillus methanolicus. BMC Microbiol 14 7.Li M, et al. (2019) Transketolase deficiency protects the liver from DNA damage by increasing levels of ribose 5-phosphate and nucleotides. Cancer Res 79 (14): 3689-3701.