Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant TTN, Human.)
Rabbit anti-Human Titin (TTN) Polyclonal Antibody | anti-TTN antibody
Polyclonal Antibody to Titin (TTN)
Immunohistochemistry: 5-20 ug/mL;
Immunocytochemistry: 5-20 ug/mL;
Optimal working dilutions must be determined by end user.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant TTN, Human.)
Western Blot (WB)
(Figure. Western Blot; Sample: Recombinant TTN, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Skeletal muscle Tissue;Primary Ab: 208g/ml Rabbit Anti- Human TTN AntibodySecond Ab: 28g/mL HRP-Linked Caprine Anti- Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Skeletal muscle Tissue;Primary Ab: 208g/ml Rabbit Anti- Human TTN AntibodySecond Ab: 28g/mL HRP-Linked Caprine Anti- Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochmistry (IHC)
(DAB staining on IHC-P; Samples:Human Cardiac Muscle Tissue; Primary Ab: 208g/ml Rabbit Anti-Human TTN AntibodySecond Ab: 28g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
Immunohistochmistry (IHC)
(DAB staining on IHC-P; Samples:Human Cardiac Muscle Tissue; Primary Ab: 208g/ml Rabbit Anti-Human TTN AntibodySecond Ab: 28g/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody(Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]
Uniprot Description
Titin: a protein kinase that is abundant in striated muscle. Is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. A N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere respectively so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Mutations are associated with familial hypertrophic cardiomyopathy 9 and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma.
Protein type: CAMK group; EC 2.7.11.1; Kinase, protein; MLCK family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 2q31.2
Cellular Component: condensed nuclear chromosome; cytosol; extracellular exosome; extracellular region; I band; M band; muscle myosin complex; Z disc
Molecular Function: actin filament binding; actinin binding; ATP binding; calcium ion binding; calmodulin binding; enzyme binding; identical protein binding; muscle alpha-actinin binding; protease binding; protein binding; protein kinase binding; protein self-association; protein serine/threonine kinase activity; protein tyrosine kinase activity; structural constituent of muscle; telethonin binding
Biological Process: cardiac muscle contraction; cardiac muscle fiber development; cardiac muscle hypertrophy; cardiac muscle morphogensis; cardiac myofibril assembly; detection of muscle stretch; mitotic chromosome condensation; muscle contraction; muscle filament sliding; muscle thin filament assembly; peptidyl-tyrosine phosphorylation; platelet degranulation; positive regulation of gene expression; positive regulation of protein secretion; protein kinase A signaling; regulation of catalytic activity; regulation of protein kinase activity; response to calcium ion; sarcomere organization; sarcomerogenesis; skeletal muscle myosin thick filament assembly; striated muscle contraction
Disease: Cardiomyopathy, Dilated, 1g; Hereditary Myopathy With Early Respiratory Failure; Muscular Dystrophy, Limb-girdle, Type 2j; Myopathy, Early-onset, With Fatal Cardiomyopathy; Tibial Muscular Dystrophy, Tardive
Research Articles on TTN
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Product Notes
The TTN ttn (Catalog #AAA2004003) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Titin (TTN) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Titin (TTN) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB), Immunoprecipitation (IP). Western blotting: 0.5-2 ug/mL; Immunohistochemistry: 5-20 ug/mL; Immunocytochemistry: 5-20 ug/mL; Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the TTN ttn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Titin (TTN), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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