Rabbit TIRP Polyclonal Antibody | anti-TICAM2 antibody

TIRP Antibody

Cat. Num. AAA150354

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TIRP Antibody is Ion exchange chromatography purified.
• Synonyms: TIRP; Polyclonal Antibody; TIRP Antibody; TRAM; TIRAP3; MyD88-4; TICAM-2; TIR domain-containing adapter molecule 2; Putative NF-kappa-B-activating protein 502; toll-like receptor adaptor molecule 2; anti-TICAM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TIRP Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 235

• Applicable Applications for anti-TICAM2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TIRP antibody can be used for detection of TIRP by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TIRP antibody was raised against a peptide corresponding to amino acids near the C-terminus of human TIRP.
• Buffer: TIRP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TIRP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TIRP in human (A), mouse (B), and rat (C) kidney cell lysates with TIRP antibody (C2) at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of mouse kidney using TIRP antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TIRP in Mouse Kidney cells with TIRP antibody at 20 μg/mL.)

Related Product Information for anti-TICAM2 antibody

TIRP Antibody: TIRP is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TIRP, along with other molecules such as TRIF, MAL, and MyD88, serves as an adaptor protein that allows for the interaction and activation of the IL-1R-associated kinase (IRAK) family, the subsequent activation of TNF receptor associated factor (TRAF)-6, and ultimately the activation of NF-κB. Expression of TIRP appears to be essential for TLR4 signalling.

NCBI and Uniprot Product Information

• NCBI GI #: 32435943
• NCBI GeneID: 100302736
• UniProt Accession #: Q86XR7; Q6JUT2; B3Y698
• Molecular Weight: 46,173 Da
• NCBI Official Full Name: TIR domain-containing adapter protein
• NCBI Official Synonym Full Names: TMED7-TICAM2 readthrough
• NCBI Official Symbol: TMED7-TICAM2
• NCBI Protein Information: TRAM adaptor with GOLD domain; TMED7-TICAM2 read-through transcript
• UniProt Protein Name: TIR domain-containing adapter molecule 2
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM2
• UniProt Synonym Gene Names: TIRAP3; TIRP; TRAM; TICAM-2; MyD88-4
• UniProt Entry Name: TCAM2_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring transmembrane emp24 protein transport domain containing 7 (TMED7) and toll-like receptor adaptor molecule 2 (TICAM2) genes. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. This fusion product functions to negatively regulate the adaptor MyD88-independent toll-like receptor 4 pathway. [provided by RefSeq, Nov 2010]

Uniprot Description

TICAM2: a toll-like receptor adapter that participates in induction of type 1 interferons. Transiently phosphorylated on serine-16 during LPS/Toll-like receptor 4 (TLR4) signaling. Activated TLR4 associates with four adapter proteins, MyD88, TIRAP, TICAM-1 (Trif), and TICAM-2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q23.1

Cellular Component: Golgi apparatus; late endosome membrane; endoplasmic reticulum; early endosome membrane; early endosome; late endosome; plasma membrane; endosome membrane

Molecular Function: signal transducer activity; phospholipid binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon-gamma production; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; positive regulation of interleukin-6 production; innate immune response; toll-like receptor 3 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway