Rabbit TIRAP Polyclonal Antibody | anti-TIRAP antibody

TIRAP (MAL, Toll-interleukin 1 Receptor Domain containing Adaptor Protein, TIRAP) (FITC)

Cat. Num. AAA6502677

• Reactivity: Mouse, Human, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TIRAP; Polyclonal Antibody; TIRAP (MAL; Toll-interleukin 1 Receptor Domain containing Adaptor Protein; TIRAP) (FITC); anti-TIRAP antibody

• Host: Rabbit
• Reactivity: Mouse, Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse TIRAP (toll-interleukin 1 receptor domain containing adaptor protein), also known as Mal. Species Crossreactivity: human and rat.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-TIRAP antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 2ug/ml; WB: 0.5-2ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region within the C-Terminus of mouse TIRAP.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of TIRAP in human heart cell lysates with MBS612536 at (A) 0.5, (B) 1, and (C) 2ug/ml.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human heart cells using T5500-02at 2ug/ml.)

Related Product Information for anti-TIRAP antibody

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.

Product Categories/Family for anti-TIRAP antibody

Antibodies; Toll Like Receptors (TLR)

References

1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.