Rabbit TIF1alpha Polyclonal Antibody | anti-TIF1alpha antibody

TIF1alpha (Q1034) Polyclonal Antibody

Cat. Num. AAA3002631

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TIF1alpha; Polyclonal Antibody; TIF1alpha (Q1034) Polyclonal Antibody; Transcription intermediary factor 1-alpha; TIF1-alpha; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24; TRIM24; RNF82; TIF1; TIF1A; anti-TIF1alpha antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TIF1alpha (Q1034) polyclonal antibody detects endogenous levels of TIF1alpha protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1016

• Applicable Applications for anti-TIF1alpha antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1011-1060 of Human TIF1alpha.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of TIF1alpha (Q1034) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-TIF1alpha antibody

The protein mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains (a RING, a B box type 1 and a B box type 2) and a coiled coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 47419909
• NCBI Accession #: NP_003843.3
• NCBI GenBank Nucleotide #: NP_003843.3
• UniProt Accession #: O15164
• Molecular Weight: ~ 117kDa
• NCBI Official Full Name: transcription intermediary factor 1-alpha isoform b
• UniProt Protein Name: Transcription intermediary factor 1-alpha
• UniProt Gene Name: TRIM24
• UniProt Synonym Gene Names: RNF82; TIF1; TIF1A; TIF1-alpha
• UniProt Entry Name: TIF1A_HUMAN

Uniprot Description

TRIM24: an atypical protein kinase that functions as a transcriptional coactivator. Originally identified as a mammalian protein that enhances the AF-2 activity of the retinoid X receptor (RXR) in a yeast genetic screen. Binds to nuclear receptors in an agonist and AF2-activating domain (AF2-AD) dependent manner. Binding to the agonist-nuclear receptor complex induces its own hyperphosphorylation as well as the phosphorylation of TFIIEa, TAFII28, and TAFII55. Belongs to a family of nuclear proteins that includes TIF1b and TIF1g. TIF1 proteins are characterized by an N-terminal region that contains a RING finger-B boxes-coiled coil (RBCC) motif, a poorly conserved central region, a C-terminal region that contains a PHD finger, and a bromodomain. Interacts with the heterochromatin-associated proteins HP1a, MOD1 (HP1b), and MOD2 (HP1g) and with the transcriptional repression domain KRAB, which is present in many Kruppel-type (C2H2) zinc finger proteins. May act as a repressor through the formation of transcriptionally inactive heterochromatin. Two splice variant isoforms have been described.

Protein type: Tumor suppressor; EC 6.3.2.-; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; Protein kinase, atypical; Kinase, protein; Ubiquitin conjugating system; ATYPICAL group; TIF1 family

Chromosomal Location of Human Ortholog: 7q32-q34

Cellular Component: nucleoplasm; perichromatin fibrils; cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; zinc ion binding; p53 binding; sequence-specific DNA binding; transcription coactivator activity; ubiquitin-protein ligase activity; estrogen response element binding; chromatin binding; methylated histone residue binding; receptor binding; ligase activity; protein kinase activity

Biological Process: calcium ion homeostasis; transcription from RNA polymerase II promoter; regulation of apoptosis; negative regulation of cell proliferation; response to peptide hormone stimulus; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; regulation of protein stability; protein ubiquitination; protein catabolic process; negative regulation of transcription, DNA-dependent

Disease: Thyroid Carcinoma, Papillary