Rabbit anti-Human TIF1 alpha Polyclonal Antibody | anti-TIF1alpha antibody
TIF1 alpha Antibody
Gene Names
RRN3; TIFIA; A-270G1.2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
TIF1 alpha; Polyclonal Antibody; TIF1 alpha Antibody; AL023001; DKFZp566E104; E130302O19Rik; MGC104238; R75565; RGD1305001; RNA polymerase I-specific transcription initiation factor RRN3; Rrn3; RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae); RRN3_HUMAN; TIF-IA; TIF1A; TIFIA; Transcription Initiation Factor 1A; Transcription initiation factor IA; Transcription initiation factor TIF IA; anti-TIF1alpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TIF1 alpha Antibody detects endogenous levels of total TIF1 alpha.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TIF1alpha antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human TIF1 alpha.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation is required for participation in rDNA transcription (By similarity). Phosphorylated at Thr-200 by MAPK9/JNK2, which abrogates initiation complex formation.
Subunit Structure
Interacts with TWISTNB, EIF3L, TAF1B and TAF1C.
Similarity
Belongs to the RRN3 family.
Subcellular Location
Nucleus>Nucleolus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-TIF1alpha antibody
Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,107 Da
NCBI Official Full Name
RNA polymerase I-specific transcription initiation factor RRN3
NCBI Official Synonym Full Names
RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)
NCBI Official Symbol
RRN3
NCBI Official Synonym Symbols
TIFIA; A-270G1.2
NCBI Protein Information
RNA polymerase I-specific transcription initiation factor RRN3; TIF-IA; transcription initiation factor IA; transcription initiation factor TIF-IA
UniProt Protein Name
RNA polymerase I-specific transcription initiation factor RRN3
UniProt Gene Name
RRN3
UniProt Synonym Gene Names
TIFIA; TIF-IA
UniProt Entry Name
RRN3_HUMAN
Uniprot Description
TIF1A: a transcription initiation factor. RNA polymerase I transcription factor RRN3. A growth-dependent regulator of ribosomal RNA synthesis
Protein type: Transcription initiation complex; Nucleolus
Chromosomal Location of Human Ortholog: 16p12
Cellular Component: nucleoplasm; nucleolus; nucleus
Biological Process: cell proliferation; in utero embryonic development; positive regulation of transcription, DNA-dependent; ribosome biogenesis and assembly; homeostasis of number of cells; transcription from RNA polymerase I promoter; gene expression; cytoplasm organization and biogenesis; transcription initiation from RNA polymerase I promoter; nucleolus organization and biogenesis
Research Articles on TIF1alpha
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Product Notes
The TIF1alpha rrn3 (Catalog #AAA9613795) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIF1 alpha Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIF1 alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the TIF1alpha rrn3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIF1 alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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