Western Blot (WB)
(All lanes : Anti-TIEG Antibody (N-term) at 1:2000 dilutionLane 1: 293T whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: human kidney lysatesLane 4: human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human TIEG Polyclonal Antibody | anti-KLF10 antibody
TIEG Antibody (N-term)
Western Blot (WB)
(All lanes : Anti-TIEG Antibody (N-term) at 1:2000 dilutionLane 1: 293T whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: human kidney lysatesLane 4: human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-TIEG Antibody (N-term) at 1:2000 dilutionLane 1: 293T whole cell lysatesLane 2: MCF-7 whole cell lysatesLane 3: human kidney lysatesLane 4: human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of anti-TIEG Antibody (N-term) pre-incubated with and without blocking peptide in CEM cell line lysate. TIEG(N-term)(arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of anti-TIEG Antibody (N-term) pre-incubated with and without blocking peptide in CEM cell line lysate. TIEG(N-term)(arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with TIEG antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Ivanov,S.V., Biochem. Biophys. Res. Commun. 370 (4), 536-540 (2008)
Jin,W., FEBS Lett. 581 (20), 3826-3832 (2007)
Fueki,N., Respiration 74 (4), 454-459 (2007)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Uniprot Description
KLF10: Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'. Belongs to the Sp1 C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding; C2H2-type zinc finger protein
Chromosomal Location of Human Ortholog: 8q22.2
Cellular Component: nucleus
Molecular Function: protein binding; metal ion binding; transcription factor activity
Biological Process: circadian rhythm; transcription, DNA-dependent; positive regulation of osteoclast differentiation; somatic stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; cellular response to starvation; regulation of circadian rhythm; bone mineralization; cell proliferation; negative regulation of cell proliferation; cell-cell signaling; transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; skeletal development
Research Articles on KLF10
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Product Notes
The KLF10 klf10 (Catalog #AAA9213102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 13-40. The TIEG Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TIEG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the KLF10 klf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIEG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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