Rabbit anti-Human Tie2 Polyclonal Antibody | anti-TEK antibody

Tie2 (Tunica Internal Endothelial Cell Kinase, Angiopoietin1 Receptor, p140 Tek, Tyrosine Protein Kinase Receptor)

Cat. Num. AAA615356

• Gene Names: TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B
• Reactivity: Human
• Applications: ELISA
• Purity: Serum; Serum
• Synonyms: Tie2; Polyclonal Antibody; Tie2 (Tunica Internal Endothelial Cell Kinase; Angiopoietin1 Receptor; p140 Tek; Tyrosine Protein Kinase Receptor); Anti -Tie2 (Tunica Internal Endothelial Cell Kinase; anti-TEK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Tie2.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.

• Applicable Applications for anti-TEK antibody: ELISA (EL/EIA)
• Application Notes: Dilution: ELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well.
• Immunogen: Synthetic peptide corresponding to 18aa, ~N-terminal, extracellular of human Tie2.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TEK antibody

Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). A family of receptor tyrosine kinases TIE1 and TIE 2 or Tek has been identified in vascular endothelium and hematopoietic cells. Mice lacking TIE 1 or TIE 2 are lethal. Ties may represent the earliest endothelial cell lineage marker and may regulate the endothelial cell proliferation, differentiation, and proper patterning during vasculogenesis. TIEs appear to be acting downstream of the VEGFRs. Tie-2 (human 1124 AA; mouse 1122 aa) is a type 1 membrane receptor protein specifically expressed in developing vascular endothelial cells and their progenitors, angioblasts. It is also found in placenta and lung, with lower levels in umbilical vein endothelial cells, brain and kidney. Tie-2 extracellular portion (25-279 aa) contains 3 fibronectin type III-like and 2-Ig-like C2-type, and 3-EGF-like domains.

Product Categories/Family for anti-TEK antibody

Antibodies; Abs to Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 112180440
• NCBI GeneID: 7010
• UniProt Accession #: Q02763; Q5TCU2; Q8IV34; A8K6W0; B4DH20; B4DHD3; D3DRK5; E7EWI2
• Molecular Weight: 125,830 Da
• NCBI Official Full Name: TEK tyrosine kinase, endothelial
• NCBI Official Synonym Full Names: TEK tyrosine kinase, endothelial
• NCBI Official Symbol: TEK
• NCBI Official Synonym Symbols: TIE2; VMCM; TIE-2; VMCM1; CD202B
• NCBI Protein Information: angiopoietin-1 receptor; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine kinase with Ig and EGF homology domains-2
• UniProt Protein Name: Angiopoietin-1 receptor
• Protein Family: Angiopoietin-1 receptor
• UniProt Gene Name: TEK
• UniProt Synonym Gene Names: TIE2; VMCM; VMCM1; hTIE2
• UniProt Entry Name: TIE2_HUMAN

NCBI Description

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations; the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

Protein type: Protein kinase, tyrosine (receptor); EC 2.7.10.1; Kinase, protein; Protein kinase, TK; Membrane protein, integral; TK group; Tie family

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: microvillus; focal adhesion; cell surface; integral to plasma membrane; basolateral plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; stress fiber; basal plasma membrane; plasma membrane

Molecular Function: protein binding; growth factor binding; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity

Biological Process: response to peptide hormone stimulus; response to cAMP; peptidyl-tyrosine phosphorylation; heart development; cell-matrix adhesion; protein amino acid autophosphorylation; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; cell-cell signaling; positive regulation of focal adhesion formation; angiogenesis; endochondral ossification; organ regeneration; Tie receptor signaling pathway; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; negative regulation of angiogenesis; positive regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; endothelial cell proliferation; negative regulation of inflammatory response; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Venous Malformations, Multiple Cutaneous And Mucosal