Rabbit anti-Human, Mouse TIE Polyclonal Antibody | anti-TIE antibody

TIE, NT (Tyrosine-protein Kinase Receptor Tie-1, TIE1) (MaxLight 405)

Cat. Num. AAA6502657

• Gene Names: TIE1; TIE; JTK14
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TIE; Polyclonal Antibody; NT (Tyrosine-protein Kinase Receptor Tie-1; TIE1) (MaxLight 405); anti-TIE antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TIE. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-TIE antibody: Immunohistochemistry (IHC), Western Blot (WB), FLISA
• Application Notes: IHC: 1:10-1:50; WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 12-42 in the N-terminal region of human TIE, UniProt Accession #P35590.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Product Categories/Family for anti-TIE antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Verstovsek, S., et al., Cancer 94(5):1517-1521 (2002). 2. Tsiamis, A.C., et al., Microvasc. Res. 63(2):149-158 (2002). 3. Sato, T.N., et al., Nature 376(6535):70-74 (1995). 4. Partanen, J., et al., Mol. Cell. Biol. 12(4):1698-1707 (1992).

NCBI and Uniprot Product Information

• NCBI GI #: 4885631
• NCBI GeneID: 7075
• NCBI Accession #: NP_005415.1
• NCBI GenBank Nucleotide #: NM_005424.4
• UniProt Accession #: P35590
• Molecular Weight: 125,090 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1 isoform 1
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: TIE1
• NCBI Official Synonym Symbols: TIE; JTK14
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• UniProt Gene Name: TIE1
• UniProt Synonym Gene Names: TIE
• UniProt Entry Name: TIE1_HUMAN

NCBI Description

This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Protein type: Kinase, protein; Protein kinase, TK; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: negative regulation of angiogenesis; peptidyl-tyrosine phosphorylation; response to retinoic acid; in utero embryonic development; mesoderm development; angiogenesis; signal transduction; vasculogenesis; negative regulation of cell migration; plasma membrane fusion