Rabbit anti-Human, Mouse TIE Polyclonal Antibody | anti-TIE1 antibody

TIE, NT (Tyrosine-protein Kinase Receptor Tie-1, TIE1)

Cat. Num. AAA6002450

• Gene Names: TIE1; TIE; JTK14
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: TIE; Polyclonal Antibody; NT (Tyrosine-protein Kinase Receptor Tie-1; TIE1); Anti -TIE; anti-TIE1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TIE. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-TIE1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the N-terminal region of human TIE (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MBS6002450 in mouse bladder tissue lysates (35ug/lane). TIE (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6002450, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Related Product Information for anti-TIE1 antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-TIE1 antibody

Antibodies; Abs to Receptors; Tyrosine Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 358356428
• NCBI GeneID: 7075
• NCBI Accession #: NP_001240286.1
• NCBI GenBank Nucleotide #: NM_001253357.1
• UniProt Accession #: P35590; B5A949; B5A950
• Molecular Weight: 125,090 Da
• NCBI Official Full Name: tyrosine-protein kinase receptor Tie-1 isoform 2
• NCBI Official Synonym Full Names: tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• NCBI Official Symbol: TIE1
• NCBI Official Synonym Symbols: TIE; JTK14
• NCBI Protein Information: tyrosine-protein kinase receptor Tie-1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1
• UniProt Protein Name: Tyrosine-protein kinase receptor Tie-1
• Protein Family: Tyrosine-protein kinase receptor
• UniProt Gene Name: TIE1
• UniProt Synonym Gene Names: TIE
• UniProt Entry Name: TIE1_HUMAN

NCBI Description

This gene encodes a member of the tyrosine protein kinase family. The encoded protein plays a critical role in angiogenesis and blood vessel stability by inhibiting angiopoietin 1 signaling through the endothelial receptor tyrosine kinase Tie2. Ectodomain cleavage of the encoded protein relieves inhibition of Tie2 and is mediated by multiple factors including vascular endothelial growth factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

TIE1: Transmembrane tyrosine-protein kinase that may modulate TEK/TIE2 activity and contribute to the regulation of angiogenesis. Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.

Protein type: Protein kinase, TK; Kinase, protein; Membrane protein, integral; EC 2.7.10.1; Protein kinase, tyrosine (receptor); TK group; Tie family

Chromosomal Location of Human Ortholog: 1p34-p33

Cellular Component: integral to plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine kinase activity; ATP binding

Biological Process: negative regulation of angiogenesis; response to retinoic acid; peptidyl-tyrosine phosphorylation; in utero embryonic development; mesoderm development; angiogenesis; signal transduction; vasculogenesis; negative regulation of cell migration; plasma membrane fusion