Rabbit anti-Human TICAM2 Polyclonal Antibody | anti-TICAM2 antibody

TICAM2 Antibody

Cat. Num. AAA9629257

• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: TICAM2; Polyclonal Antibody; TICAM2 Antibody; Putative NF-kappa-B-activating protein 502; TCAM2_HUMAN; TICAM-2; TIR containing adapter molecule 2; TIR domain containing adaptor protein; TIR domain-containing adapter molecule 2; TIRAP 3; TIRAP3; TIRAP3a short form; TIRP; Toll-like receptor adaptor protein 3; Toll/interleukin-1 receptor domain-containing protein; TRAM; TRIF-related adapter molecule; anti-TICAM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: TICAM2 Antibody detects endogenous levels of total TICAM2.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TICAM2 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The TIR domain mediates the interaction with TRAF6 and MYD88.
• Post Translational Modifications: Phosphorylated by PRKCE in response to LPS. Phosphorylation is essential for its function. It is depleted from the membrane upon phosphorylation. Tyrosine phosphorylation is inhibited by phosphatase PTPN4.Isoform 1 is myristoylated. Required for membrane association which is critical for its ability to initiate efficient signaling.
• Subunit Structure: Homodimer. Interacts with TLR4, TICAM1, IRF3 and IRF7 in response to LPS. Interacts with IL1R1, IL1RAP, IRAK2, IRAK3 and TRAF6. Interacts with protein kinase-inactive mutants of IRAK1 and IRAK4. Isoform 1 interacts with isoform 2; the interaction occurs in late endosomes and disrupts the interaction between isoform 1 and TICAM1. Interacts with MYD88; the interaction decreases after IL-18 stimulation in a time-dependent manner. Interacts with IL18R1 and IL18RAP. Interacts with TLR2. Interacts with RAB11FIP2.
• Tissue Specificity: Expressed in spleen, prostate, testis, uterus, small intestine, colon, peripheral blood leukocytes, heart, placenta, lung, liver, skeletal muscle, and pancreas Isoform 2 is ubiquitously expressed (at lower levels than isoform 1).
• Function: Functions as sorting adapter in different signaling pathways to facilitate downstream signaling leading to type I interferon induction. In TLR4 signaling, physically bridges TLR4 and TICAM1 and functionally transmits signal to TICAM1 in early endosomes after endocytosis of TLR4. In TLR2 signaling, physically bridges TLR2 and MYD88 and is required for the TLR2-dependent movement of MYD88 to endosomes following ligand engagement. Involved in IL-18 signaling and is proposed to function as a sorting adapter for MYD88 in IL-18 signaling during adaptive immune response. Forms a complex with RAB11FIP2 that is recruited to the phagosomes to promote the activation of the actin-regulatory GTPases RAC1 and CDC42 and subsequent phagocytosis of Gram-negative bacteria.Proposed to inhibit LPS-TLR4 signaling at the late endosome by interaction with isoform 1 thereby disrupting the association of isoform 1 with TICAM1. May be involved in TLR4 degradation in late endosomes.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-TICAM2 antibody

Cytoplasm; Golgi apparatus; Cell membrane; Endoplasmic reticulum; Early endosome membrane; Late endosome membrane; Cell projection; Phagocytic cup; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 256985100
• NCBI GeneID: 100302736
• NCBI Accession #: NP_001157940.1
• NCBI GenBank Nucleotide #: NM_001164468.3
• UniProt Accession #: Q86XR7; Q6JUT2; B3Y698
• Molecular Weight: 46,173 Da
• NCBI Official Full Name: TRAM adaptor with GOLD domain isoform 1
• NCBI Official Synonym Full Names: TMED7-TICAM2 readthrough
• NCBI Official Symbol: TMED7-TICAM2
• NCBI Protein Information: TRAM adaptor with GOLD domain; TMED7-TICAM2 read-through transcript
• UniProt Protein Name: TIR domain-containing adapter molecule 2
• Protein Family: TIR domain-containing adapter molecule
• UniProt Gene Name: TICAM2
• UniProt Synonym Gene Names: TIRAP3; TIRP; TRAM; TICAM-2; MyD88-4
• UniProt Entry Name: TCAM2_HUMAN

NCBI Description

This locus represents naturally occurring read-through transcription between the neighboring transmembrane emp24 protein transport domain containing 7 (TMED7) and toll-like receptor adaptor molecule 2 (TICAM2) genes. Alternative splicing results in multiple transcript variants, one of which encodes a fusion protein that shares sequence identity with the products of each individual gene. This fusion product functions to negatively regulate the adaptor MyD88-independent toll-like receptor 4 pathway. [provided by RefSeq, Nov 2010]

Uniprot Description

TICAM2: a toll-like receptor adapter that participates in induction of type 1 interferons. Transiently phosphorylated on serine-16 during LPS/Toll-like receptor 4 (TLR4) signaling. Activated TLR4 associates with four adapter proteins, MyD88, TIRAP, TICAM-1 (Trif), and TICAM-2.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q23.1

Cellular Component: Golgi apparatus; early endosome membrane; endoplasmic reticulum; late endosome membrane; early endosome; late endosome; plasma membrane; endosome membrane

Molecular Function: signal transducer activity; phospholipid binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interferon-gamma production; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; negative regulation of toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of interleukin-6 production; innate immune response; toll-like receptor 3 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway