Rabbit anti-Rat Thyroid Iodide Transporter Polyclonal Antibody | anti-Tpo antibody

Thyroid Iodide Transporter (TIT)

Cat. Num. AAA611227

• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Thyroid Iodide Transporter; Polyclonal Antibody; Thyroid Iodide Transporter (TIT); Anti -Thyroid Iodide Transporter (TIT); anti-Tpo antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat Thyroid Iodide Transporter. No significant sequence homology is seen with glucose transporter or other Na+-dependent transporters.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 40% glycerol.

• Applicable Applications for anti-Tpo antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.; Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml.; Western Blot: 1-10ug/ml (ECL); A band of 95-110kD has been detected in brush border membrane (2).; Immunofluorescence: 2-20ug/ml; Immunohistochemistry: 2-20ug/ml
• Immunogen: A 16 AA peptide sequence, near the C-terminal, cytoplasmic domain of rat TIT (1) was synthesized, coupled to KLH
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Tpo antibody

Iodide (I-) is an essential constituent of thyroid hormones. Thyroid epithelial cells for the biosynthesis of thyroid hormones actively transport it. At the cellular level, Iodide transport is mediated by intrinsic membrane Na+/I+ symporter (NIS/TIT). Rat TIT/SLC5A (618aa; ~66kD; mouse 618aa; human 643aa) is predicted to contain 12 transmembrane domains. The N-terminus has a short extracellular domain, whereas C-terminus is predicted to be cytoplasmic. TIT is primarily expressed in thyroid but also detected in mammary gland and ovary. Its expression is reduced in tumors. Defects in TIT gene are associated with iodide transport defect (ITD), which is characterized by an inability of the thyroid to maintain concentration difference of readily exchangeable iodine between plasma and the thyroid gland. It is a cause of congenital hypothyroidism.

Product Categories/Family for anti-Tpo antibody

Antibodies; Abs to Transport Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 402766537
• NCBI GeneID: 54314
• NCBI Accession #: NP_062226.2
• NCBI GenBank Nucleotide #: NM_019353.2
• UniProt Accession #: P14650
• Molecular Weight: 101,460 Da
• NCBI Official Full Name: thyroid peroxidase
• NCBI Official Synonym Full Names: thyroid peroxidase
• NCBI Official Symbol: Tpo
• NCBI Protein Information: thyroid peroxidase
• UniProt Protein Name: Thyroid peroxidase
• Protein Family: Thyroid peroxidase
• UniProt Gene Name: Tpo
• UniProt Synonym Gene Names: TPO
• UniProt Entry Name: PERT_RAT

NCBI Description

membrane-bound glycoprotein; iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4 [RGD, Feb 2006]

Uniprot Description

Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4.

Catalytic activity: 2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O.[Thyroglobulin]-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3-iodo-L-tyrosine + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + iodide + H2O2 = [thyroglobulin]-3,5-diiodo-L-tyrosine + 2 H2O.2 [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-L-thyroxine + [thyroglobulin]-aminoacrylate + 2 H2O.[Thyroglobulin]-3-iodo-L-tyrosine + [thyroglobulin]-3,5-diiodo-L-tyrosine + H2O2 = [thyroglobulin]-3,5,3'-triiodo-L-thyronine + [thyroglobulin]-aminoacrylate + 2 H2O.

Cofactor: Binds 1 calcium ion per heterodimer

By similarity.Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer

By similarity.

Pathway: Hormone biosynthesis; thyroid hormone biosynthesis.

Subunit structure: Interacts with DUOX1, DUOX2 and CYBA

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Post-translational modification: Heme is covalently bound through a H2O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface

By similarity.Cleaved in its N-terminal part

By similarity.

Sequence similarities: Belongs to the peroxidase family. XPO subfamily.Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.