Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Thrombin Activatable Fibrinolysis Inhibitor (TAFI) Polyclonal Antibody | anti-TAFI antibody
Polyclonal Antibody to Thrombin Activatable Fibrinolysis Inhibitor (TAFI)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Thrombin Activatable Fibrinolysis Inhibitor (TAFI); Polyclonal Antibody; Polyclonal Antibody to Thrombin Activatable Fibrinolysis Inhibitor (TAFI); anti-TAFI antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TAFI. It has been selected for its ability to recognize TAFI in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
0.42mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-GKEHRVK NAIWIDCGIH AREWISPAFC LWFIGYVTQF HGKENTYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSVHMNNRCV GTDLNRNFAS KHWCEKGASS FSCSETYCGL YPESEPEVKA VADFLRRNIN HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERFI KPTCAEALAA VSKIAWH
MGHHHHHHSGSEF-GKEHRVK NAIWIDCGIH AREWISPAFC LWFIGYVTQF HGKENTYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSVHMNNRCV GTDLNRNFAS KHWCEKGASS FSCSETYCGL YPESEPEVKA VADFLRRNIN HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERFI KPTCAEALAA VSKIAWH
Sequence Length
3
Applicable Applications for anti-TAFI antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TAFI (Gly164~His417) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2045066
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,827 Da
NCBI Official Full Name
carboxypeptidase B2
NCBI Official Synonym Full Names
carboxypeptidase B2
NCBI Official Symbol
Cpb2
NCBI Protein Information
carboxypeptidase B2
UniProt Protein Name
Carboxypeptidase B2
UniProt Gene Name
Cpb2
UniProt Synonym Gene Names
CPR; CPU; TAFI
NCBI Description
a thrombin-activatable fibrinolysis inhibitor [RGD, Feb 2006]
Uniprot Description
CPB2: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. Belongs to the peptidase M14 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.20; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q11
Cellular Component: extracellular space
Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity; zinc ion binding
Biological Process: blood coagulation; fibrinolysis; negative regulation of fibrinolysis; proteolysis; response to drug; response to heat
Research Articles on TAFI
Similar Products
Product Notes
The TAFI cpb2 (Catalog #AAA2003008) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Thrombin Activatable Fibrinolysis Inhibitor (TAFI) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Thrombin Activatable Fibrinolysis Inhibitor (TAFI) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TAFI cpb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-GKEHRV K NAIWIDCGIH AREWISPAFC LWFIGYVTQF HGKENTYTRL LRHVDFYIMP VMNVDGYDYT WKKNRMWRKN RSVHMNNRCV GTDLNRNFAS KHWCEKGASS FSCSETYCGL YPESEPEVKA VADFLRRNIN HIKAYISMHS YSQQILFPYS YNRSKSKDHE ELSLVASEAV RAIESINKNT RYTHGSGSES LYLAPGGSDD WIYDLGIKYS FTIELRDTGR YGFLLPERFI KPTCAEALAA VSKIAWH. It is sometimes possible for the material contained within the vial of "Thrombin Activatable Fibrinolysis Inhibitor (TAFI), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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