Rabbit anti-Human THOC1 Polyclonal Antibody | anti-THOC1 antibody

THOC1, CT (THOC1, HPR1, THO complex subunit 1, Nuclear matrix protein p84, hTREX84) (Azide free) (HRP)

Cat. Num. AAA6355430

• Gene Names: THOC1; P84; HPR1; P84N5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: THOC1; Polyclonal Antibody; CT (THOC1; HPR1; THO complex subunit 1; Nuclear matrix protein p84; hTREX84) (Azide free) (HRP); hTREX84; anti-THOC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-THOC1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: THOC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 540-570 amino acids from the C-terminal region of human THOC1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(THOC1 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the THOC1 antibody detected the THOC1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of THOC1 (arrow) using rabbit polyclonal THOC1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the THOC1 gene.)

Related Product Information for anti-THOC1 antibody

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).

Product Categories/Family for anti-THOC1 antibody

Antibodies; Nuclear Proteins

References

Davila, S., et al. Genes Immun. 11(3):232-238(2010). Liu, Y., et al. Mol. Psychiatry (2010). In press: Boyne, J.R., et al. PLoS Pathog. 4 (10), E1000194 (2008): Ferreira, M.A., et al. Nat. Genet. 40(9):1056-1058(2008). Yang, J., et al. Ann. Clin. Lab. Sci. 38(2):105-112(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 154448889
• NCBI GeneID: 9984
• NCBI Accession #: NP_005122.2
• NCBI GenBank Nucleotide #: NM_005131
• UniProt Accession #: Q96FV9; Q15219; Q64I72; Q64I73; B2RBP6
• Molecular Weight: 43,359 Da
• NCBI Official Full Name: Homo sapiens THO complex 1 (THOC1), mRNA
• NCBI Official Synonym Full Names: THO complex 1
• NCBI Official Symbol: THOC1
• NCBI Official Synonym Symbols: P84; HPR1; P84N5
• NCBI Protein Information: THO complex subunit 1; hTREX84; nuclear matrix protein p84; tho1
• UniProt Protein Name: THO complex subunit 1
• UniProt Gene Name: THOC1
• UniProt Synonym Gene Names: HPR1; Tho1; p84N5
• UniProt Entry Name: THOC1_HUMAN

NCBI Description

HPR1 is part of the TREX (transcription/export) complex, which includes TEX1 (MIM 606929), THO2 (MIM 300395), ALY (MIM 604171), and UAP56 (MIM 142560).[supplied by OMIM, Nov 2010]

Uniprot Description

Tho1: a protein associated the nuclear matrix. Part of the TREX (transcription/export) complex. The TREX complex is recruited to transcribed genes and travels with the RNA polymerase during elongation. It may physically link proteins that function in transcription and in RNA export.

Protein type: Spliceosome

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: nucleoplasm; intercellular bridge; nuclear matrix; cytoplasm; nuclear speck; nucleus

Molecular Function: protein binding; DNA binding; RNA binding

Biological Process: RNA processing; mRNA export from nucleus; transcription, DNA-dependent; negative regulation of isotype switching to IgA isotypes; apoptosis; RNA splicing; positive regulation of RNA elongation; regulation of DNA recombination; intronless viral mRNA export from host nucleus; signal transduction; mRNA processing; regulation of RNA elongation; replication fork processing