Rabbit anti-Human, Mouse TGIF2 Polyclonal Antibody | anti-TGIF2 antibody

TGIF2, CT (TGIF2, Homeobox protein TGIF2, 5'-TG-3'-interacting factor 2, TGF-beta-induced transcription factor 2) (Azide free) (HRP)

Cat. Num. AAA6355265

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TGIF2; Polyclonal Antibody; CT (TGIF2; Homeobox protein TGIF2; 5'-TG-3'-interacting factor 2; TGF-beta-induced transcription factor 2) (Azide free) (HRP); TGF-beta-induced transcription factor 2; anti-TGIF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TGIF2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TGIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-199 amino acids from the C-terminal region of human TGIF2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TGIF2 Antibody (C-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TGIF2 antibody detected the TGIF2 protein (arrow).)

Flow Cytometry (FC/FACS)

(TGIF2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TGIF2 antibody

The protein encoded by this gene is a DNA-binding homeobox protein and a transcriptional repressor. The encoded protein appears to repress transcription by recruiting histone deacetylases to TGF beta-responsive genes. This gene is amplified and overexpressed in some ovarian cancers, and mutations in this gene can cause holoprosencephaly.

Product Categories/Family for anti-TGIF2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Watanabe, T., et al. Jpn. J. Cancer Res. 93(10):1114-1122(2002). Deloukas, P., et al. Nature 414(6866):865-871(2001). Melhuish, T.A., et al. J. Biol. Chem. 276(34):32109-32114(2001). Imoto, I., et al. Biochem. Biophys. Res. Commun. 276(1):264-270(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 11141881
• NCBI GeneID: 60436
• NCBI Accession #: NP_068581.1
• NCBI GenBank Nucleotide #: NM_021809.6
• UniProt Accession #: Q9GZN2; B2R9U3; E1P5T9; H0YNI0
• Molecular Weight: 17,908 Da
• NCBI Official Full Name: homeobox protein TGIF2
• NCBI Official Synonym Full Names: TGFB-induced factor homeobox 2
• NCBI Official Symbol: TGIF2
• NCBI Protein Information: homeobox protein TGIF2; 5'-TG-3' interacting factor 2; TGF-beta-induced transcription factor 2; TGF(beta)-induced transcription factor 2; TGFB-induced factor 2 (TALE family homeobox); transcription growth factor-beta-induced factor 2
• UniProt Protein Name: Homeobox protein TGIF2
• Protein Family: Homeobox protein
• UniProt Gene Name: TGIF2
• UniProt Synonym Gene Names: TGFB-induced factor 2
• UniProt Entry Name: TGIF2_HUMAN

NCBI Description

The protein encoded by this gene is a DNA-binding homeobox protein and a transcriptional repressor, which appears to repress transcription by recruiting histone deacetylases to TGF beta-responsive genes. This gene is amplified and over-expressed in some ovarian cancers. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. Read-through transcription also exists between this gene and the neighboring downstream C20orf24 (chromosome 20 open reading frame 24) gene. [provided by RefSeq, Dec 2010]

Uniprot Description

TGIF2: Transcriptional repressor, which probably repress transcription by binding directly the 5'-CTGTCAA-3' DNA sequence or by interacting with TGF-beta activated SMAD proteins. Probably represses transcription via the recruitment of histone deacetylase proteins. Belongs to the TALE/TGIF homeobox family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: nucleoplasm; centrosome; nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription, DNA-dependent; retina development in camera-type eye; transforming growth factor beta receptor signaling pathway; regulation of gastrulation; gene expression; negative regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation