Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human TGF-beta Receptor I Polyclonal Antibody | anti-TGFBR1 antibody
Rabbit polyclonal to Human TGF-beta Receptor I
Gene Names
TGFBR1; AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
TGF-beta Receptor I; Polyclonal Antibody; Rabbit polyclonal to Human TGF-beta Receptor I; TGF-beta Receptor I Rabbit pab Antibody; anti-TGFBR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Sequence Length
426
Applicable Applications for anti-TGFBR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1/200-500; IHC: 1/50-100
Immunogen
A synthetic peptide of human TGF-beta Receptor I
Storage Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TGFBR1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-TGFBR1 antibody
Transforming growth factor-beta (TGF-beta) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-4). TGF-beta elicits signaling through three cell surface receptors: type I (RI), type II (RII) and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases (5). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-beta with high affinity but lacks a cytoplasmic signaling domain (6,7). Expression of the type III receptor can regulate TGF-beta signaling through presentation of the ligand to the signaling complex. The only known direct TGF-beta signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription (8,9).
References
1. Massague, J. et al. (2000) Cell 103, 295-309.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
2. Caestecker, M.P. et al. (2000) J. Natl. Cancer Inst. 92, 1388-1402.
3. Derynck, R. et al. (2001) Nature Genet. 29, 117-129.
4. Miyazono, K. et al. (2000) Adv. Immunol. 75, 115-157.
5. Derynck, R. et al. (1997) Biochim. Biophys. Acta. 1333, F105-150.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,960 Da
NCBI Official Full Name
TGF-beta receptor type-1 isoform 2
NCBI Official Synonym Full Names
transforming growth factor, beta receptor 1
NCBI Official Symbol
TGFBR1
NCBI Official Synonym Symbols
AAT5; ALK5; MSSE; SKR4; ALK-5; LDS1A; LDS2A; TGFR-1; ACVRLK4
NCBI Protein Information
TGF-beta receptor type-1; tbetaR-I; TGF-beta receptor type I; TGF-beta type I receptor; activin receptor-like kinase 5; transforming growth factor beta receptor I; serine/threonine-protein kinase receptor R4; activin A receptor type II-like kinase, 53kD; activin A receptor type II-like kinase, 53kDa; transforming growth factor-beta receptor type I; activin A receptor type II-like protein kinase of 53kD; transforming growth factor, beta receptor I (activin A receptor type II-like kinase, 53kD)
UniProt Protein Name
TGF-beta receptor type-1
Protein Family
UniProt Gene Name
TGFBR1
UniProt Synonym Gene Names
ALK5; SKR4; TGFR-1; ALK-5; ALK5; SKR4; TGF-beta receptor type I; TbetaR-I
UniProt Entry Name
TGFR1_HUMAN
Similar Products
Product Notes
The TGFBR1 tgfbr1 (Catalog #AAA126054) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit polyclonal to Human TGF-beta Receptor I reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TGF-beta Receptor I can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1/200-500; IHC: 1/50-100. Researchers should empirically determine the suitability of the TGFBR1 tgfbr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF-beta Receptor I, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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