Western Blot (WB)
(Western blot analysis of TFIIH p89 expression in MCF7 (A), mouse brain (B) whole cell lysates.)
Rabbit anti-Human, Mouse TFIIH Polyclonal Antibody | anti-TFIIH antibody
Anti-TFIIH p89 Antibody
IHC: 1:50-1:200
Western Blot (WB)
(Western blot analysis of TFIIH p89 expression in MCF7 (A), mouse brain (B) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TFIIH p89 expression in MCF7 (A), mouse brain (B) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TFIIH p89 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of TFIIH p89 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an ATP-dependent DNA helicase that functions in nucleotide excision repair. The encoded protein is a subunit of basal transcription factor 2 (TFIIH) and, therefore, also functions in class II transcription. Mutations in this gene are associated with Xeroderma pigmentosum B, Cockayne's syndrome, and trichothiodystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Uniprot Description
XPB: ATP-dependent 3'-5' DNA helicase, component of the core- TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. Acts by opening DNA either around the RNA transcription start site or the DNA damage. One of the 6 subunits forming the core-TFIIH basal transcription factor which associates with the CAK complex composed of CDK7, CCNH/cyclin H and MNAT1 to form the TFIIH basal transcription factor. Interacts with PUF60. Interacts with ATF7IP. Interacts with Epstein-Barr virus EBNA2. Belongs to the helicase family. RAD25/XPB subfamily.
Protein type: Helicase; DNA repair, damage; EC 3.6.4.12; Transcription factor
Chromosomal Location of Human Ortholog: 2q21
Cellular Component: nucleoplasm; holo TFIIH complex; nucleus
Molecular Function: protein C-terminus binding; DNA-dependent ATPase activity; GTP binding; ATPase activity; dATP binding; 3'-5' DNA helicase activity; protein N-terminus binding; peptide binding; transcription factor binding; protein kinase activity; ATP-dependent DNA helicase activity; RNA polymerase subunit kinase activity; protein binding; DNA binding; damaged DNA binding; ATP binding
Biological Process: transcription from RNA polymerase II promoter; DNA topological change; viral reproduction; apoptosis; positive regulation of apoptosis; positive regulation of viral transcription; protein amino acid phosphorylation; regulation of gene expression, epigenetic; protein localization; mRNA capping; UV protection; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; nucleotide-excision repair, DNA damage removal; response to UV; transcription initiation from RNA polymerase II promoter; hair cell differentiation; nucleotide-excision repair, DNA incision; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; termination of RNA polymerase I transcription; DNA repair; nucleotide-excision repair, DNA duplex unwinding; nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; response to hypoxia; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; transcription initiation from RNA polymerase I promoter
Disease: Trichothiodystrophy 2, Photosensitive; Xeroderma Pigmentosum, Complementation Group B
Research Articles on TFIIH
Similar Products
Product Notes
The TFIIH ercc3 (Catalog #AAA8306592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TFIIH p89 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFIIH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the TFIIH ercc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFIIH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
