Rabbit TFIIF Polyclonal Antibody | anti-GTF2F2 antibody
TFIIF Antibody
Gene Names
GTF2F2; BTF4; RAP30; TF2F2; TFIIF
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TFIIF; Polyclonal Antibody; TFIIF Antibody; ATP dependent helicase GTF2F2; ATP-dependent helicase GTF2F2; BTF4; General transcription factor IIF 30 kDa subunit; General transcription factor IIF polypeptide 2; General transcription factor IIF subunit 2; General transcription factor IIF; polypeptide 2; 30kDa; Gtf2f2; RAP30; T2FB_HUMAN; TF2F2; TFIIF beta; TFIIF-beta; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30; anti-GTF2F2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TFIIF Antibody detects endogenous levels of total TFIIF.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GTF2F2 antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from Human MYOCD.
Conjugation
Unconjugated
Similarity
Belongs to the TFIIF beta subunit family.
Subunit Structure
Heterodimer of an alpha and a beta subunit. Interacts with HTATSF1 and GPBP1 (By similarity). Interacts with URI1. Interacts with GTF2B (via N-terminus); this interaction is inhibited in presence of GTF2F1.
Function
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,380 Da
NCBI Official Full Name
general transcription factor IIF subunit 2
NCBI Official Synonym Full Names
general transcription factor IIF, polypeptide 2, 30kDa
NCBI Official Symbol
GTF2F2
NCBI Official Synonym Symbols
BTF4; RAP30; TF2F2; TFIIF
NCBI Protein Information
general transcription factor IIF subunit 2; ATP-dependent helicase GTF2F2; TFIIF-beta; general transcription factor IIF 30 kDa subunit; transcription initiation factor IIF subunit beta; transcription initiation factor RAP30
UniProt Protein Name
General transcription factor IIF subunit 2
Protein Family
UniProt Gene Name
GTF2F2
UniProt Synonym Gene Names
RAP30; TFIIF-beta
UniProt Entry Name
T2FB_HUMAN
Uniprot Description
GTF2F2: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA- helicase activity. Belongs to the TFIIF beta subunit family.
Protein type: Transcription initiation complex; Nuclear receptor co-regulator; EC 3.6.4.12; Helicase
Chromosomal Location of Human Ortholog: 13q14
Cellular Component: nucleoplasm; microtubule cytoskeleton; transcription factor TFIIF complex; nucleus
Molecular Function: protein binding; DNA binding; ATP-dependent helicase activity; ATP binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; mRNA capping; regulation of transcription, DNA-dependent; viral reproduction; positive regulation of viral transcription; RNA splicing; RNA elongation from RNA polymerase II promoter; gene expression
Research Articles on GTF2F2
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Product Notes
The GTF2F2 gtf2f2 (Catalog #AAA9629266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TFIIF Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TFIIF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the GTF2F2 gtf2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFIIF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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