Rabbit TFEB Polyclonal Antibody | anti-TFEB antibody

Phospho-TFEB (Ser142) Antibody

Cat. Num. AAA9612913

• Gene Names: TFEB; TCFEB; BHLHE35; ALPHATFEB
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: TFEB; Polyclonal Antibody; Phospho-TFEB (Ser142) Antibody; Alpha TFEB; AlphaTFEB; bHLHe35; Class E basic helix-loop-helix protein 35; T cell transcription factor EB; TCFEB; TFEB_HUMAN; Transcription factor EB; anti-TFEB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-TFEB (Ser142) Antibody detects endogenous levels of TFEB only when phosphorylated at Ser142.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-TFEB antibody: Western Blot (WB), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human TFEB around the phosphorylation site of Ser142.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Sumoylated; does not affect dimerization with MITF.
• Subunit Structure: Homodimer and heterodimer; with TFE3 or MITF.
• Similarity: The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.Belongs to the MiT/TFE family.
• Subcellular Location: Cytoplasm. Nucleus.; Note: Mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). The translocation to the nucleus is regulated by ATP13A2 (PubMed:23434374, PubMed:27278822). In macrophages, translocates into the nucleus upon live S.enterica infection (PubMed:27184844).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Related Product Information for anti-TFEB antibody

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4 (+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Regulates lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1.

NCBI and Uniprot Product Information

• NCBI GI #: 429836865
• NCBI GeneID: 7942
• NCBI Accession #: NP_001161299.2
• NCBI GenBank Nucleotide #: NM_001167827.2
• UniProt Accession #: P19484; Q709B3; Q7Z6P9; Q9BRJ5; Q9UJD8
• Molecular Weight: 52 KD
• NCBI Official Full Name: transcription factor EB isoform 2
• NCBI Official Synonym Full Names: transcription factor EB
• NCBI Official Symbol: TFEB
• NCBI Official Synonym Symbols: TCFEB; BHLHE35; ALPHATFEB
• NCBI Protein Information: transcription factor EB; T-cell transcription factor EB; class E basic helix-loop-helix protein 35
• UniProt Protein Name: Transcription factor EB
• Protein Family: Transcription factor
• UniProt Gene Name: TFEB
• UniProt Synonym Gene Names: BHLHE35; bHLHe35
• UniProt Entry Name: TFEB_HUMAN

Uniprot Description

TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: embryonic placenta development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; lysosome organization and biogenesis; autophagy; positive regulation of transcription from RNA polymerase II promoter; humoral immune response; positive regulation of autophagy