Rabbit anti-Human TFE3 Polyclonal Antibody | anti-TFE3 antibody

TFE3 Rabbit pAb

Cat. Num. AAA4757101

• Gene Names: TFE3; TFEA; RCCP2; RCCX1; bHLHe33
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot
• Purity: Affinity purified
• Synonyms: TFE3; Polyclonal Antibody; TFE3 Rabbit pAb; Transcription factor E3; transcription factor binding to IGHM enhancer 3; TFEA; RCCP2; RCCX1; bHLHe33; anti-TFE3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Ubiquitous in fetal and adult tissues. Gene expression databases
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine(pH7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-TFE3 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB)
• Application Notes: WB: 1:1000; IHC-P: 1:50-1:200; ICC/IF: 1:20-1:50; FC/FACS: 1:20
• Immunogen: Recombinant protein of human TFE3
• Conjugation: Unconjugated
• Modification: Unmodified
• Cell Position: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot detection of TFE3 in Hela,293T,Raw264.7, Jurkat using TFE3 Rabbit pAb.Predicted band size:62kDa.Observed band size:75,80kDa.)

Related Product Information for anti-TFE3 antibody

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.
This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Product Categories/Family for anti-TFE3 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 8659574
• NCBI GeneID: 7030
• NCBI Accession #: NP_006512.2
• NCBI GenBank Nucleotide #: NM_006521.4
• UniProt Accession #: P19532; Q92757; Q92758; Q99964; A8MZL6
• Molecular Weight: 61,521 Da
• NCBI Official Full Name: transcription factor E3
• NCBI Official Synonym Full Names: transcription factor binding to IGHM enhancer 3
• NCBI Official Symbol: TFE3
• NCBI Official Synonym Symbols: TFEA; RCCP2; RCCX1; bHLHe33
• NCBI Protein Information: transcription factor E3; Transcription factor for IgH enhancer; transcription factor E family, member A; class E basic helix-loop-helix protein 33
• UniProt Protein Name: Transcription factor E3
• Protein Family: Transcription factor
• UniProt Gene Name: TFE3
• UniProt Synonym Gene Names: BHLHE33; bHLHe33
• UniProt Entry Name: TFE3_HUMAN

NCBI Description

The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1); it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb)-TFE3 and ASPL-TFE3.[provided by RefSeq, Dec 2009]

Uniprot Description

TFE3: Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. A chromosomal aberration involving TFE3 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with ASPSCR1 forms a ASPSCR1-TFE3 fusion protein. Chromosomal aberrations involving TFE3 are found in patients with papillary renal cell carcinoma. Translocation t(X;1)(p11.2;q21.2) with PRCC; translocation t(X;1)(p11.2;p34) with PSF; inversion inv(X)(p11.2;q12) that fuses NONO to TFE3. Belongs to the MiT/TFE family.

Protein type: Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: Xp11.22

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein dimerization activity; transcription factor activity

Biological Process: regulation of osteoclast differentiation; positive regulation of cell adhesion; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; humoral immune response

Disease: Renal Cell Carcinoma, Xp11-associated