Rabbit TEM2 Polyclonal Antibody | anti-RASD2 antibody

TEM2 Antibody

Cat. Num. AAA150695

• Gene Names: RASD2; Rhes; TEM2; MGC:4834
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TEM2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TEM2; Polyclonal Antibody; TEM2 Antibody; Rhes; MGC:4834; GTP-binding protein Rhes; Ras homolog enriched in striatum; RASD family; member 2; anti-RASD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: TEM2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 266

• Applicable Applications for anti-RASD2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TEM2 antibody can be used for detection of TEM2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TEM2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human TEM2.
• Buffer: TEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TEM2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TEM2 in human colon tissue lysate with TEM2 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of TEM2 in human colon tissue with TEM2 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TEM2 in Human Colon cells with TEM2 antibody at 20 μg/mL.)

Related Product Information for anti-RASD2 antibody

TEM2 Antibody: Rhes, also known as tumor endothelial marker 2 (TEM2), is a small GTP-binding protein that is predominantly expressed in the striatal region of the brain. This protein belongs to the RASD subfamily of Ras-related GTP-binding protein superfamily and is thought to play a role in the normal development and function of the brain as mice lacking this gene showed increased anxiety levels and motor coordination deficits. Rhes was identified as TEM2 through analysis of genes whose expression was upregulated in tumor endothelium. Tumor endothelial markers are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies.

NCBI and Uniprot Product Information

• NCBI GI #: 21362868
• NCBI GeneID: 23551
• UniProt Accession #: Q96D21; O95520; Q5THY8
• Molecular Weight: 30,366 Da
• NCBI Official Full Name: GTP-binding protein Rhes
• NCBI Official Synonym Full Names: RASD family, member 2
• NCBI Official Symbol: RASD2
• NCBI Official Synonym Symbols: Rhes; TEM2; MGC:4834
• NCBI Protein Information: GTP-binding protein Rhes; tumor endothelial marker 2; Ras homolog enriched in striatum
• UniProt Protein Name: GTP-binding protein Rhes
• Protein Family: GTP-binding protein
• UniProt Gene Name: RASD2
• UniProt Synonym Gene Names: TEM2
• UniProt Entry Name: RHES_HUMAN

NCBI Description

This gene encodes a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades. [provided by RefSeq, Jul 2008]

Uniprot Description

RASD2: GTPase signaling protein that binds to and hydrolyzes GTP. Regulates signaling pathways involving G-proteins-coupled receptor and heterotrimeric proteins such as GNB1, GNB2 and GNB3. May be involved in selected striatal competencies, mainly locomotor activity and motor coordination. Belongs to the small GTPase superfamily. RasD family.

Protein type: G protein, monomeric, RasD; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: plasma membrane

Molecular Function: GTPase activity; GTP binding; ubiquitin conjugating enzyme binding; G-protein beta-subunit binding

Biological Process: positive regulation of protein sumoylation; synaptic transmission, dopaminergic; positive regulation of protein kinase B signaling cascade; metabolic process; small GTPase mediated signal transduction; locomotory behavior; negative regulation of protein ubiquitination