Rabbit anti-Mouse Telomeric Repeat Binding Factor 2 Polyclonal Antibody | anti-TERF2 antibody

Telomeric Repeat Binding Factor 2 (TRF2, RAP2)

Cat. Num. AAA617062

• Gene Names: TERF2; TRF2; TRBF2
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Telomeric Repeat Binding Factor 2; Polyclonal Antibody; Telomeric Repeat Binding Factor 2 (TRF2; RAP2); Anti -Telomeric Repeat Binding Factor 2 (TRF2; anti-TERF2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: The 21aa mouse control peptide has no significant sequence homology with TRF1. mouse TRF21 has 73% sequence homology with the human TRF2.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-TERF2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: A 21aa Peptide sequence near the C-terminus of Mouse TRF2
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TERF2 antibody

The 3'ends of chromosomes are capped with telomere sequences (TTAGGG; 6-26 nucleotides in length) by ribonucleoprotein telomerase during DNA replication. Telomerase is an unusual RNA-dependent DNA polymerase that uses and RNA component to specify the addition of telomere. The telomeric RNA contains a sequence complementary to TTAGGG. In ciliated protozoa and yeast, telomere length is maintained by regulating the activity of telomerase. Many mammalian cells do not express telomerase resulting into shortening of telomere with each cell division, and ultimately causing the chromosomal instability, aging and cell death. Approx. 4.8 kb of telomeric DNA is lost with each cell division resulting into large number of chromosomal abnormalities.

Purification of telomerase from the ciliate also revealed two protein of 43 and 123 kD. p123 is a homolog of yeast Est2 (Essential for Telomerase activity). Mammalian homologs of yeast Est2 (also known as TP2 for Telomerase associated Protein 2; hEST2 or telomerase catalytic subunit or telomerase reverse transcriptase, TERT) have also been cloned (human Est2, 1132 aa; mouse Est2 1122 aa; ~127 kD). (1).
Telomeric Repeat binding Factors (TRF1 and TRF2) bind the telomeric TTAGGG repeat. TRFs have been colocalized with telomeric DNA in metaphase cells. TRFs are located at chromosome ends during metaphase. Mouse and human TRF2 are 495 aa and 500 aa, respectively. TRF1 and TRF2 share ~30% homology.

Product Categories/Family for anti-TERF2 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5032169
• NCBI GeneID: 7014
• NCBI Accession #: NP_005643.1
• NCBI GenBank Nucleotide #: NM_005652.3
• UniProt Accession #: Q15554
• Molecular Weight: 55,551 Da
• NCBI Official Full Name: telomeric repeat-binding factor 2
• NCBI Official Synonym Full Names: telomeric repeat binding factor 2
• NCBI Official Symbol: TERF2
• NCBI Official Synonym Symbols: TRF2; TRBF2
• NCBI Protein Information: telomeric repeat-binding factor 2; OTTHUMP00000174887; telomeric DNA-binding protein; TTAGGG repeat-binding factor 2; telomeric repeat binding protein 2
• UniProt Protein Name: Telomeric repeat-binding factor 2
• Protein Family: Telomeric repeat-binding factor
• UniProt Gene Name: TERF2
• UniProt Synonym Gene Names: TRBF2; TRF2
• UniProt Entry Name: TERF2_HUMAN

NCBI Description

This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq]

Uniprot Description

TRF2: Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single- stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology- directed repair (HDR), which can affect telomere length. Homodimer. Component of the shelterin complex (telosome) composed of TERF1, TERF2, TINF2, TERF2IP/RAP1, ACD and POT1. Interacts with TERF2IP. Interacts with NBN. Interacts with SLX4/BTBD12. Interacts with DCLRE1B/Apollo and TERF2IP/RAP1; the interaction is direct. Ubiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; DNA repair, damage

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: male germ cell nucleus; nucleoplasm; Golgi apparatus; chromosome, telomeric region; nuclear telomere cap complex; Mre11 complex; nuclear chromosome, telomeric region; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; protein binding; protein homodimerization activity; telomeric DNA binding; double-stranded telomeric DNA binding; chromatin binding

Biological Process: negative regulation of telomere maintenance; telomere capping; in utero embryonic development; negative regulation of telomere maintenance via semi-conservative replication; telomeric loop formation; positive regulation of nitric-oxide synthase activity; positive regulation of telomere maintenance; telomere maintenance via telomerase; age-dependent telomere shortening; cell cycle; telomere maintenance; protection from non-homologous end joining at telomere