anti-Human Telomeric Repeat Binding Factor 1 Polyclonal Antibody | anti-TERF1 antibody
Anti - Telomeric Repeat Binding Factor 1 (TRF1, RAP1)
Gene Names
TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; FLJ41416; hTRF1-AS
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Telomeric Repeat Binding Factor 1; Polyclonal Antibody; Anti - Telomeric Repeat Binding Factor 1 (TRF1; RAP1); Telomeric Repeat Binding Factor 1 (TRF1; anti-TERF1 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The 20 AA human control peptide has no significant sequence homology with TRF2. We recommend the use for the use mouse TRF1. Actual crossreactivity of antibodies in various species has not been studied
Purity/Purification
Serum
Form/Format
Serum
Supplied as a liquid, neat serum, 40% glycerol.
Supplied as a liquid, neat serum, 40% glycerol.
Sequence Length
439
Applicable Applications for anti-TERF1 antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2).
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified.
Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 20 AA Peptide sequence near the C-terminus of human TRF1 (2). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-TERF1 antibody
TRF1 is a negative regulator of telomere length maintenance (1). TRF1 has a number of biochemical similarities to Rap1p, a distantly related DNA-binding protein that functions at telomeres in yeast (1,2). Like Rap1p, TRF1 requires two Myb motifs for DNA binding. Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (3,4). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. Recently, it has been shown that TRF1 expression is significantly elevated in patients with acute lymphoblastic leukemia (ALL) compared to those in acute myeloid leukemia (AML) (5). In this study, TRF1 expression tended to be higher in patients without telomere shortening and in those with hTERT expression suggesting that TRF1 may act to monitor telomere length under the condition of up-regulated telomerase activity in some neoplastic cells.
Product Categories/Family for anti-TERF1 antibody
References
• 1. Bianchi A, Smith S, Chong L, Elias P, de Lange T. EMBO J 16
• 7. :1785-94 (1997).
• 2. Broccoli D, Smogorzewska A, Chong L, de Lange T. Nat Genet 17
• 2. :231-235 (1997).
• 3. Smith et al., Science 282:1484-1487, 1998.
• 4. Smith S, de Lange T. J Cell Sci 112: 3649-56, 1999.
• 5. Kim S-h, Kaminker P, and Campisi J. Nat. Genet. 23: 405 - 412 (1999).
• 6. Ohyashiki JH, Hayashi S, Yahata N, Iwama H, Ando K, Tauchi T, Ohyashiki K. Int J Oncol 18(3):593- 598 (2001). 7. Closed chromatin loops at the ends of chromosomes. Tatiana Nikitina and Christopher L. Woodcock. J. Cell Biol., Jul 2004; 10.1083/jcb.200403118.
• 8. Chong L et al. A human telomeric protein. Science 270 :1663-7 (1995). PubMed: 7502076.9. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review).10. Chong L et al (1995) Science 270, 1663-1667; Broccoli D et al Gene accession # P70731.
• 7. :1785-94 (1997).
• 2. Broccoli D, Smogorzewska A, Chong L, de Lange T. Nat Genet 17
• 2. :231-235 (1997).
• 3. Smith et al., Science 282:1484-1487, 1998.
• 4. Smith S, de Lange T. J Cell Sci 112: 3649-56, 1999.
• 5. Kim S-h, Kaminker P, and Campisi J. Nat. Genet. 23: 405 - 412 (1999).
• 6. Ohyashiki JH, Hayashi S, Yahata N, Iwama H, Ando K, Tauchi T, Ohyashiki K. Int J Oncol 18(3):593- 598 (2001). 7. Closed chromatin loops at the ends of chromosomes. Tatiana Nikitina and Christopher L. Woodcock. J. Cell Biol., Jul 2004; 10.1083/jcb.200403118.
• 8. Chong L et al. A human telomeric protein. Science 270 :1663-7 (1995). PubMed: 7502076.9. Meyerson M et al (1997) Cell 90, 785-795; Greenberg RA et al (1998) Oncogene in press; Nugent CI et al (1998) Genes Develop. 12, 1073-85 (review).10. Chong L et al (1995) Science 270, 1663-1667; Broccoli D et al Gene accession # P70731.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,246 Da
NCBI Official Full Name
telomeric repeat-binding factor 1 isoform 1
NCBI Official Synonym Full Names
telomeric repeat binding factor (NIMA-interacting) 1
NCBI Official Symbol
TERF1
NCBI Official Synonym Symbols
TRF; PIN2; TRF1; TRBF1; t-TRF1; FLJ41416; hTRF1-AS
NCBI Protein Information
telomeric repeat-binding factor 1; OTTHUMP00000226889; OTTHUMP00000226890; NIMA-interacting protein 2; telomeric protein Pin2/TRF1; TTAGGG repeat-binding factor 1
UniProt Protein Name
Telomeric repeat-binding factor 1
Protein Family
UniProt Gene Name
TERF1
UniProt Synonym Gene Names
PIN2; TRBF1; TRF; TRF1
UniProt Entry Name
TERF1_HUMAN
Similar Products
Product Notes
The TERF1 terf1 (Catalog #AAA619717) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - Telomeric Repeat Binding Factor 1 (TRF1, RAP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Telomeric Repeat Binding Factor 1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. A band of 95-110kD has been detected in brush border membrane (2). ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the TERF1 terf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Telomeric Repeat Binding Factor 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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