Rabbit anti-Human TELO2 Polyclonal Antibody | anti-TELO2 antibody

TELO2, CT (TELO2, KIAA0683, Telomere length regulation protein TEL2 homolog, Protein clk-2 homolog) (Azide free) (HRP)

Cat. Num. AAA6354803

• Gene Names: TELO2; CLK2; TEL2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TELO2; Polyclonal Antibody; CT (TELO2; KIAA0683; Telomere length regulation protein TEL2 homolog; Protein clk-2 homolog) (Azide free) (HRP); Protein clk-2 homolog; anti-TELO2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TELO2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TELO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 792~822 amino acids from the C-terminal region of human TELO2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TELO2 Antibody (C-term) in K562 cell line lysates (35ug/lane). TELO2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TELO2 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TELO2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TELO2 Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TELO2 antibody

TELO2 is a protein that functions as an S-phase checkpoint protein in the cell cycle. The protein may also play a role in DNA repair.

Product Categories/Family for anti-TELO2 antibody

Antibodies; KIAA; orf

References

Collis, S.J., et al. Mol. Cell 32(3):313-324(2008). Takai, H., et al. Cell 131(7):1248-1259(2007). Collis, S.J., et al. Nat. Cell Biol. 9(4):391-401(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 166987394
• NCBI GeneID: 9894
• UniProt Accession #: Q9Y4R8; O75168; Q7LDV4; Q9BR21; D3DU73
• Molecular Weight: 837
• NCBI Official Full Name: Telomere length regulation protein TEL2 homolog
• NCBI Official Synonym Full Names: telomere maintenance 2
• NCBI Official Symbol: TELO2
• NCBI Official Synonym Symbols: CLK2; TEL2
• NCBI Protein Information: telomere length regulation protein TEL2 homolog; protein clk-2 homolog; TEL2, telomere maintenance 2, homolog
• UniProt Protein Name: Telomere length regulation protein TEL2 homolog
• Protein Family: TELO2-interacting protein
• UniProt Gene Name: TELO2
• UniProt Synonym Gene Names: KIAA0683; hCLK2
• UniProt Entry Name: TELO2_HUMAN

NCBI Description

This gene encodes a protein that functions as an S-phase checkpoint protein in the cell cycle. The protein may also play a role in DNA repair.[provided by RefSeq, Mar 2009]

Uniprot Description

TELO2: Regulator of the DNA damage response (DDR). Part of the TTT complex that is required to stabilize protein levels of the phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins. The TTT complex is involved in the cellular resistance to DNA damage stresses, like ionizing radiation (IR), ultraviolet (UV) and mitomycin C (MMC). Together with the TTT complex and HSP90 may participate in the proper folding of newly synthesized PIKKs. Promotes assembly, stabilizes and maintains the activity of mTORC1 and mTORC2 complexes, which regulate cell growth and survival in response to nutrient and hormonal signals. May be involved in telomere length regulation. Component of the TTT complex composed of TELO2, TTI1 and TTI2. Interacts with ATM, ATR, MTOR, PRKDC, RUVBL2, TTI1, TTI2, SMG1 and TRRAP. Belongs to the TEL2 family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: chromosome, telomeric region; membrane; cytoplasm; TORC2 complex; intracellular; nucleus

Molecular Function: protein binding; protein complex binding; protein complex scaffold

Biological Process: regulation of TOR signaling pathway