Rabbit anti-Human TCFL5 Polyclonal Antibody | anti-TCFL5 antibody

TCFL5, CT (TCFL5, CHA, E2BP1, Transcription factor-like 5 protein, Cha transcription factor, HPV-16 E2-binding protein 1)

Cat. Num. AAA646273

• Gene Names: TCFL5; CHA; Figlb; E2BP-1; bHLHe82
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TCFL5; Polyclonal Antibody; CT (TCFL5; CHA; E2BP1; Transcription factor-like 5 protein; Cha transcription factor; HPV-16 E2-binding protein 1); Anti -TCFL5; anti-TCFL5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-TCFL5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: TCFL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-411 amino acids from the C-terminal region of human TCFL5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TCFL5 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the TCFL5 antibody detected the TCFL5 protein (arrow).)

Related Product Information for anti-TCFL5 antibody

Putative transcription factor. Isoform 3 may play a role in early spermatogenesis.

Product Categories/Family for anti-TCFL5 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 38505159
• NCBI GeneID: 10732
• NCBI Accession #: NP_006593.2
• NCBI GenBank Nucleotide #: NM_006602.2
• UniProt Accession #: Q9UL49; O94771; Q9BYW0
• Molecular Weight: 52,697 Da
• NCBI Official Full Name: transcription factor-like 5 protein
• NCBI Official Synonym Full Names: transcription factor-like 5 (basic helix-loop-helix)
• NCBI Official Symbol: TCFL5
• NCBI Official Synonym Symbols: CHA; Figlb; E2BP-1; bHLHe82
• NCBI Protein Information: transcription factor-like 5 protein; cha transcription factor; HPV-16 E2 binding protein 1; HPV-16 E2-binding protein 1
• UniProt Protein Name: Transcription factor-like 5 protein
• Protein Family: Transcription factor-like 5 protein
• UniProt Gene Name: TCFL5
• UniProt Synonym Gene Names: CHA; E2BP1; E2BP-1
• UniProt Entry Name: TCFL5_HUMAN

Uniprot Description

Function: Putative transcription factor. Isoform 3 may play a role in early spermatogenesis. Ref.4

Subunit structure: Efficient DNA binding requires dimerization with another bHLH protein.

Subcellular location: Nucleus Ref.4.

Tissue specificity: Isoform 3 is testis specific. Isoform 2 is pancreas specific. Ref.4

Developmental stage: Isoform 3 is specifically expressed in primary spermatocytes at the pachytene stage, but not those at leptonema stage. Not expressed in other testicular cells, including spermatogonia located in the basal compartment of the seminiferous tubule or spermatids. Ref.4

Miscellaneous: Antibodies against TCFL5 are present in sera from patients with Chagas' disease, an autoimmune disease caused by Trypanosoma cruzi. Two different epitopes that mimic Trypanosoma cruzi antigens have been identified: R1 and R3 epitopes, which are recognized by T- and B-cells, respectively.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Sequence caution: The sequence BAA36557.1 differs from that shown. Reason: Erroneous initiation.