Rabbit anti-Human TCEB2 Polyclonal Antibody | anti-TCEB2 antibody

TCEB2, ID (TCEB2, Transcription elongation factor B polypeptide 2, Elongin 18kD subunit, Elongin-B, RNA polymerase II transcription factor SIII subunit B, SIII p18) (AP)

Cat. Num. AAA6354403

• Gene Names: TCEB2; ELOB; SIII
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TCEB2; Polyclonal Antibody; ID (TCEB2; Transcription elongation factor B polypeptide 2; Elongin 18kD subunit; Elongin-B; RNA polymerase II transcription factor SIII subunit B; SIII p18) (AP); SIII p18; anti-TCEB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TCEB2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TCEB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-48 amino acids from the Central region of human TCEB2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TCEB2 Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TCEB2 antibody detected the TCEB2 protein (arrow).)

Related Product Information for anti-TCEB2 antibody

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13.

Product Categories/Family for anti-TCEB2 antibody

Antibodies; Transcription Factors

References

Marcsisin, S.R., et al. J. Mol. Biol. 402(5):892-904(2010). Piessevaux, J., et al. J. Biol. Chem. 283(31):21334-21346(2008). Van Herreweghe, E., et al. EMBO J. 26(15):3570-3580(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Bullock, A.N., et al. Proc. Natl. Acad. Sci. U.S.A. 103(20):7637-7642(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 6005890
• NCBI GeneID: 6923
• NCBI Accession #: NP_009039.1
• NCBI GenBank Nucleotide #: NM_007108.3
• UniProt Accession #: Q15370; B7WPD3
• Molecular Weight: 17,911 Da
• NCBI Official Full Name: transcription elongation factor B polypeptide 2 isoform a
• NCBI Official Synonym Full Names: transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B)
• NCBI Official Symbol: TCEB2
• NCBI Official Synonym Symbols: ELOB; SIII
• NCBI Protein Information: transcription elongation factor B polypeptide 2; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; elongin 18 kDa subunit; elongin, 18-kD subunit; elongin-B
• UniProt Protein Name: Elongin-B
• Protein Family: Transcription elongation factor B polypeptide
• UniProt Gene Name: ELOB
• UniProt Synonym Gene Names: EloB

NCBI Description

This gene encodes the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. Pseudogenes have been identified on chromosomes 11 and 13. [provided by RefSeq, Aug 2008]

Uniprot Description

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex) (PubMed:7638163). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells ().