Rabbit anti-Human, Mouse TCEA2 Polyclonal Antibody | anti-TCEA2 antibody

TCEA2 (Transcription Elongation Factor A Protein 2, Testis-specific S-II, Transcription Elongation Factor S-II Protein 2, Transcription Elongation Factor TFIIS.l) (HRP)

Cat. Num. AAA6396152

• Gene Names: TCEA2; TFIIS
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TCEA2; Polyclonal Antibody; TCEA2 (Transcription Elongation Factor A Protein 2; Testis-specific S-II; Transcription Elongation Factor S-II Protein 2; Transcription Elongation Factor TFIIS.l) (HRP); anti-TCEA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TCEA2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TCEA2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TCEA2, aa1-299 (NP_003186.1).
• Immunogen Sequence: MMGKEEEIARIARRLDKMVTKKSAEGAMDLLRELKAMPITLHLLQSTRVGMSVNALRKQSSDEEVIALAKSLIKSWKKLLDASDAKARERGRGMPLPTSSRDASEAPDPSRKRPELPRAPSTPRITTFPPVPVTCDAVRNKCREMLTAALQTDHDHVAIGADCERLSAQIEECIFRDVGNTDMKYKNRVRSRISNLKDAKNPDLRRNVLCGAITPQQIAVMTSEEMASDELKEIRKAMTKEAIREHQMARTGGTQTDLFTCGKCRKKNCTYTQVQTRSSDEPMTTFVVCNECGNRWKFC
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TCEA2 rabbit polyclonal antibody. Western Blot analysis of TCEA2 expression in mouse testis.)

Western Blot (WB)

(Western Blot analysis of TCEA2 expression in transfected 293T cell line by TCEA2 polyclonal antibody. Lane 1: TCEA2 transfected lysate (33.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TCEA2 antibody

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TCEA2 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4507385
• NCBI GeneID: 6919
• NCBI Accession #: NP_003186.1
• NCBI GenBank Nucleotide #: NM_003195.4
• UniProt Accession #: Q15560; Q8TD37; Q8TD38; B3KNM1
• Molecular Weight: 30,569 Da
• NCBI Official Full Name: transcription elongation factor A protein 2 isoform a
• NCBI Official Synonym Full Names: transcription elongation factor A (SII), 2
• NCBI Official Symbol: TCEA2
• NCBI Official Synonym Symbols: TFIIS
• NCBI Protein Information: transcription elongation factor A protein 2; testis-specific S-II; transcription elongation factor S-II protein 2; transcription elongation factor TFIIS.1; transcription elongation factor TFIIS.l
• UniProt Protein Name: Transcription elongation factor A protein 2
• Protein Family: Tuliposide A-converting enzyme
• UniProt Gene Name: TCEA2
• UniProt Entry Name: TCEA2_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus, where it functions as an SII class transcription elongation factor. Elongation factors in this class are responsible for releasing RNA polymerase II ternary complexes from transcriptional arrest at template-encoded arresting sites. The encoded protein has been shown to interact with general transcription factor IIB, a basal transcription factor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TCEA2: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Belongs to the TFS-II family.

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; transcription elongation factor complex; centrosome

Molecular Function: protein binding; DNA binding; zinc ion binding

Biological Process: RNA elongation; positive regulation of transcription from RNA polymerase II promoter; regulation of RNA elongation