Rabbit anti-Human, Mouse TBX20 Polyclonal Antibody | anti-TBX20 antibody

TBX20, CT (TBX20, T-box transcription factor TBX20) (Azide free) (HRP)

Cat. Num. AAA6354187

• Gene Names: TBX20; ASD4
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBX20; Polyclonal Antibody; CT (TBX20; T-box transcription factor TBX20) (Azide free) (HRP); T-box transcription factor TBX20; anti-TBX20 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-TBX20 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBX20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 307-335 amino acids from the C-terminal region of human TBX20.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane). This demonstrates the TBX20 antibody detected the TBX20 protein (arrow).)

Western Blot (WB)

(Western Blot analysis using MBS641283 in mouse spleen tissue lysates (35ug/lane). This demonstrates the TBX20 antibody detected the TBX20 protein (arrow).)

Related Product Information for anti-TBX20 antibody

This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-TBX20 antibody

Antibodies; Transcription Factors

References

Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010). Posch, M.G., et al. J. Med. Genet. 47(4):230-235(2010). Macindoe, I., et al. J. Mol. Biol. 389(3):606-618(2009). Liu, C., et al. Eur J Med Genet 51(6):580-587(2008). Hammer, S., et al. J. Cell. Biochem. 104(3):1022-1033(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 261337146
• NCBI GeneID: 57057
• NCBI Accession #: NP_001159692.1
• NCBI GenBank Nucleotide #: NM_001166220.1
• UniProt Accession #: Q9UMR3; Q000T4; Q0IJ70; Q0VAS1; Q9Y2N5; A4D1Y6
• Molecular Weight: 49,232 Da
• NCBI Official Full Name: T-box transcription factor TBX20 isoform 2
• NCBI Official Synonym Full Names: T-box 20
• NCBI Official Symbol: TBX20
• NCBI Official Synonym Symbols: ASD4
• NCBI Protein Information: T-box transcription factor TBX20; T-box protein 20
• UniProt Protein Name: T-box transcription factor TBX20
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX20
• UniProt Synonym Gene Names: T-box protein 20
• UniProt Entry Name: TBX20_HUMAN

NCBI Description

This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

TBX20: Probable transcriptional regulator involved in developmental processes. Defects in TBX20 are the cause of atrial septal defect type 4 (ASD4). ASD4 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. ASD4 patients show other heart abnormalities including defects in septation, chamber growth and valvulogenesis. ASD4 is not associated with defects in the cardiac conduction system or with non-cardiac abnormalities.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: cytoplasm; nucleus

Molecular Function: transcription factor activity

Biological Process: transcription, DNA-dependent; blood circulation; lateral mesoderm formation; neuron migration; negative regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; cell proliferation; visceral motor neuron differentiation; muscle contraction; dorsal/ventral pattern formation; endoderm formation; cardiac muscle morphogensis; positive regulation of transcription from RNA polymerase II promoter; heart looping; negative regulation of transcription, DNA-dependent; positive regulation of cardiac muscle cell proliferation

Disease: Atrial Septal Defect 4