Immunohistochemistry (IHC)
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with TBC1D4 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Goat anti-Human TBC1D4 Polyclonal Antibody | anti-TBC1D4 antibody
TBC1D4, NT (TBC1 Domain Family Member 4, Akt Substrate Of 160kD, AS160, KIAA0603)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:128,000
Western Blot: 0.1-0.3ug/ml
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml
Immunohistochemistry (IHC)
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with TBC1D4 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(Human Skeletal Muscle (formalin-fixed, paraffin-embedded) stained with TBC1D4 antibody at 3.75ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)
Chemiluminescence
(Antibody (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Chemiluminescence
(Antibody (0.1ug/ml) staining of Daudi cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
AS160: a widely-expressed Rab GTPase-activating protein which apparently plays a role in mediating insulin action. Phosphorylated by the Ser/Thr protein kinase Akt in response to insulin. Is redistributed from low-density microsomes to the cytosol upon insulin treatment. Insulin stimulation of GLUT4 exocytosis is dependent on AS160. The translocation of GLUT4 from intracellular storage sites to the cell surface functions to stimulate insulin uptake by adipose and muscle tissue. Its expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects; its phosphorylation is impaired in skeletal muscle of type 2 diabetics. The protein is expressed in pancreatic Langerhans islets, including beta cells. Up-regulated in T cells from patients with atopic dermatitis. Two alternatively spliced isoforms have been described. Isoform 2, which promotes GLUT4 translocation at the plasma membrane, is the highest overexpressed in most tissues. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver or adipose tissue.
Protein type: GAPs, Rab; GAPs
Chromosomal Location of Human Ortholog: 13q22.2
Cellular Component: cytoplasmic vesicle membrane; cytoplasm
Biological Process: vesicle-mediated transport; negative regulation of vesicle fusion; cellular response to insulin stimulus
Disease: Diabetes Mellitus, Noninsulin-dependent, 5
Research Articles on TBC1D4
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Product Notes
The TBC1D4 tbc1d4 (Catalog #AAA6002997) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The TBC1D4, NT (TBC1 Domain Family Member 4, Akt Substrate Of 160kD, AS160, KIAA0603) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Western Blot and Immunohistochemistry. Dilution: ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3.75ug/ml. Researchers should empirically determine the suitability of the TBC1D4 tbc1d4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBC1D4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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