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Western Blot (WB)
(Western blot analysis of CEM cell line lysates (35ug/lane) usingMBS641579.This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).)
Rabbit anti-Human, Mouse TBC1D13 Polyclonal Antibody | anti-TBC1D13 antibody
TBC1D13, ID (TBC1D13, TBC1 domain family member 13) (Azide free) (HRP)
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TBC1D13; Polyclonal Antibody; ID (TBC1D13; TBC1 domain family member 13) (Azide free) (HRP); TBC1 domain family member 13; anti-TBC1D13 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TBC1D13. Species crossreactivity: mouse
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-TBC1D13 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 76-105 in the central region of human TBC1D13. UniProt Accession #Q9NVG8.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of CEM cell line lysates (35ug/lane) usingMBS641579.This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).)
Western Blot (WB)
(Western blot analysis of CEM cell line lysates (35ug/lane) usingMBS641579.This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).)
Western Blot (WB)
(Western blot analysis of mouse spleen tissue lysates (35ug/lane) usingMBS641579. This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).)
Western Blot (WB)
(Western blot analysis of mouse spleen tissue lysates (35ug/lane) usingMBS641579. This demonstrates the TBC1D13 antibody detected the TBC1D13 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded mouse heart tissue using MBS641579 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of formalin fixed and paraffin embedded mouse heart tissue using MBS641579 followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NCI-H460 cell using MBS641579 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of NCI-H460 cell using MBS641579 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-TBC1D13 antibody
TBC1D13 may act as a GTPase-activating protein for Rab family protein(s).
Product Categories/Family for anti-TBC1D13 antibody
References
Ishibashi, K., et al. Genes Cells 14(1):41-52(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48.9 kDa (423aa) confirmed by MALDI-TOF
NCBI Official Full Name
TBC1 domain family member 13 isoform a
NCBI Official Synonym Full Names
TBC1 domain family member 13
NCBI Official Symbol
TBC1D13
NCBI Protein Information
TBC1 domain family member 13
UniProt Protein Name
TBC1 domain family member 13
Protein Family
UniProt Gene Name
TBC1D13
UniProt Entry Name
TBC13_HUMAN
Uniprot Description
TBC1D13: a putative GTPase activating protein for Rab family protein(s).
Protein type: GAPs; GAPs, Rab
Chromosomal Location of Human Ortholog: 9q34.11
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Product Notes
The TBC1D13 tbc1d13 (Catalog #AAA6353857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TBC1D13, ID (TBC1D13, TBC1 domain family member 13) (Azide free) (HRP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TBC1D13 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TBC1D13 tbc1d13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBC1D13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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