Rabbit anti-Human, Mouse Tau Polyclonal Antibody | anti-Tau antibody

Tau Antibody: ATTO 488

Cat. Num. AAA8002068

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: Tau; Polyclonal Antibody; Tau Antibody: ATTO 488; Rabbit Anti-Human Tau Polyclonal; Tau Antibody; Neurofibrillary tangle protein Antibody; MAPTL Antibody; Microtubule-associated protein tau Antibody; MTBT1 Antibody; Paired helical filament-tau Antibody; TAU Antibody; PHF-tau Antibody; MAPT Antibody; AI413597 antibody; AW045860 antibody; DDPAC antibody; FLJ31424 antibody; FTDP 17 antibody; G protein beta1/gamma2 subunit interacting factor 1 antibody; MAPT antibody; MAPTL antibody; MGC134287 antibody; MGC138549 antibody; MGC156663 antibody; Microtubule associated protein tau antibody; Microtubule associated protein tau isoform 4 antibody; Microtubule-associated protein tau antibody; MSTD antibody; Mtapt antibody; MTBT1 antibody; MTBT2 antibody; Neurofibrillary tangle protein antibody; Paired helical filament tau antibody; Paired helical filament-tau antibody; PHF tau antibody; PHF-tau antibody; PPND antibody; PPP1R103 antibody; Protein phosphatase 1; regulatory subunit 103 antibody; pTau antibody; RNPTAU antibody; TAU antibody; TAU_HUMAN antibody; Tauopathy and respiratory failure; included antibody; anti-Tau antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Detects ~45.8kda. Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 776

• Applicable Applications for anti-Tau antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000
• Immunogen: Synthetic peptide from the full length of Human Tau
• Immunogen Species: Human
• Conjugate: ATTO 488
• Cellular Localization: Cytoskeleton, Plasma Membrane, Cell Membrane, Peripheral Membrane Protein, Cytoplasmic Side, Cytosol, Axon, Microtubule, Microtubule Associated Complex, Tubulin Complex, Nucleus, Nuclear Periphery, Nuclear Speck, Axolemma Plasma Membrane, Axolemma, Cytoplasm, Cell Body, Cytoplasmic Ribonucleoprotein Granule, Dendrite, Growth Cone, Neurofibrillary Tangle, Neuronal Cell Body
• Certificate of Analysis: A 1:1000 dilution was sufficient for detection of Tau in 10 mug of human SH-SY5Y cell lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Western Blot (WB)

(Western blot analysis of Human SH-SY5Y cell lysate showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody . Lane 1: Molecular Weight Ladder (MW). Lane 2: Human SH-SY5Y cell lysate. Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band(s): 50 kDa, 65 kDa.)

Related Product Information for anti-Tau antibody

Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing hyperphosphorylated tau fibrils (3). There are six isoforms of tau in the adult human brain: three with four repeat units (4R) and three with three repeat units (3R) (4). K18 is a truncated form of human tau containing only the 4 microtubule binding repeats (5). P301L (PL) is a mutation where proline is replaced by leucine at codon 301 of tau, and has been linked to frontotemporal dementia (6).

Product Categories/Family for anti-Tau antibody

Alzheimer's Disease; Axon Markers; Cell Markers; Cell Signaling; Cytoskeleton; Microtubules; MT Associated Proteins; Neurodegeneration; Neuron Markers; Neuroscience; Tangles & Tau

References

1. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146-148 (1907). 2. Matsumoto, G. et al. (2018). Int J Mol Sci. 19, 1497. 3. Goedert, M. and Spillantini, M. G. (2017). Mol Brain. 10:18. 4. Guo, J. and Lee, M.Y. (2013). FEBS Lett. 587(6): 717-723. 5. Alberici, A. et al. (2004). J Neurol Neurosurg Psychiatry. 75:1607-1610.

NCBI and Uniprot Product Information

• NCBI GI #: 294862258
• NCBI GeneID: 4137
• NCBI Accession #: NP_001116538.2
• NCBI GenBank Nucleotide #: NM_001123066.3
• UniProt Accession #: P10636
• NCBI Official Full Name: microtubule-associated protein tau isoform 6
• NCBI Official Synonym Full Names: microtubule associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Tautomerase
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU; PHF-tau
• UniProt Entry Name: TAU_HUMAN

NCBI Description

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: microtubule; microtubule associated complex; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; enzyme binding; structural constituent of cytoskeleton; microtubule binding; apolipoprotein binding; protein kinase binding; SH3 domain binding

Biological Process: axon extension; apoptosis; positive regulation of microtubule polymerization; positive regulation of axon extension; axon cargo transport; neuron migration; microtubule cytoskeleton organization and biogenesis; adult walking behavior; regulation of microtubule polymerization; mitochondrion transport along microtubule; negative regulation of intracellular transport; generation of neurons; regulation of autophagy; cell structure disassembly during apoptosis

Disease: Supranuclear Palsy, Progressive, 1; Pick Disease Of Brain; Frontotemporal Dementia; Parkinson-dementia Syndrome; Parkinson Disease, Late-onset; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related