Rabbit anti-Human Tau Polyclonal Antibody | anti-Tau antibody

Phospho-Tau (thr 175) Rabbit Polyclonal Antibody

Cat. Num. AAA588283

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Synonyms: Tau; Polyclonal Antibody; Phospho-Tau (thr 175) Rabbit Polyclonal Antibody; anti-Tau antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody recognizes the human Phospho-Tau protein peptide at the Threonine 175 (Thr 175) phosphorylation site. (MW 48-67 kDa)
• Form/Format: Lyophilized immunogen affinity purified in PBS pH7.4

• Applicable Applications for anti-Tau antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommend starting dilution:; If reconstituted with deionized water in 50 uL:; WB: 1:10000; IHC 1:1000; Optimal dilution has to be determined by the user.
• Immunogen: Peptide phosphorylated at Thr 175
• Target: Phospho-Tau(thr175)
• Preservative: None
• Preparation and Storage: Lyophilized antibodies can be kept at 4ºC for up to 3 months and should be kept at -20ºC for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80ºC) or kept at 4ºC for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy.

Immunohistochemistry (IHC)

(Immuonohistochemistry analysis of a competition assay demonstrating the specificity of the anti-Tau (Thr 175) antibody.)

Western Blot (WB)

(Western blot detection of insoluble phospho-Tau protein using the anti-Tau (Thr 175) antibody in samples isolated from patients with a neurodegenerative disease (Amyolropic lateral sclerosis, ALSci or Alzheimer's disease, AD).)

Related Product Information for anti-Tau antibody

Tauopathies are a group of diverse dementias and movement disorders which have a common pathological feature in that they all display the presence of intracellular accumulation of abnormal filaments of tau protein. Furthermore, these accumulated tau proteins all display an abnormal phosphorylation pattern that may be disease specific. As an example. Amyotrophic lateral sclerosis (ALS, also referred to as Charcot's disease or Lou Gehrig's disease) is the most common form of progressive motor neuron disease in North America. ALS gradually disables the patient's voluntary motor muscle control until it becomes fatal.

References

1. Gohar M-Tau phosphorylation at threonine-175 leads to fibril formation and enhanced cell death: implications for amyotrophic lateral sclerosis with cogniti. 2. Strong MJ-Tau protein hyperphosphorylation in sporadic ALS with cognitive impairment.