Rabbit anti-Human, Mouse TAPT1 Polyclonal Antibody | anti-TAPT1 antibody

TAPT1, CT (TAPT1, CMVFR, Transmembrane anterior posterior transformation protein 1 homolog, Cytomegalovirus partial fusion receptor) (AP)

Cat. Num. AAA6353578

• Gene Names: TAPT1; CMVFR
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAPT1; Polyclonal Antibody; CT (TAPT1; CMVFR; Transmembrane anterior posterior transformation protein 1 homolog; Cytomegalovirus partial fusion receptor) (AP); Cytomegalovirus partial fusion receptor; anti-TAPT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TAPT1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 533-562 amino acids from the C-terminal region of human TAPT1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAPT1 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the TAPT1 antibody detected the TAPT1 protein (arrow).)

Western Blot (WB)

(TAPT1 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TAPT1 antibody detected the TAPT1 protein (arrow).)

Flow Cytometry (FC/FACS)

(TAPT1 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TAPT1 antibody

This gene encodes a highly conserved, putative transmembrane protein. A mutation in the mouse ortholog of this gene results in homeotic, posterior-to-anterior transformations of the axial skeleton which are similar to the phenotype of mouse homeobox C8 gene mutants. This gene is proposed to function downstream of homeobox C8 to transduce extracellular patterning information during axial skeleton development. An alternatively spliced transcript variant encoding a substantially different isoform has been described, but its biological validity has not been determined.

Product Categories/Family for anti-TAPT1 antibody

Antibodies; Transmembrane Proteins

References

Howell, G.R., et al. Genetics 175(2):699-707(2007). Baldwin, B.R., et al. J. Gen. Virol. 81 (PT 1), 27-35 (2000): Baldwin, B.R., et al. Biochem. Biophys. Res. Commun. 219(2):668-673(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 130977756
• NCBI GeneID: 202018
• NCBI Accession #: NP_699196.2
• NCBI GenBank Nucleotide #: NM_153365.2
• UniProt Accession #: Q6NXT6; Q8N2S3; Q9NZK9
• Molecular Weight: 64,260 Da
• NCBI Official Full Name: transmembrane anterior posterior transformation protein 1 homolog
• NCBI Official Synonym Full Names: transmembrane anterior posterior transformation 1
• NCBI Official Symbol: TAPT1
• NCBI Official Synonym Symbols: CMVFR
• NCBI Protein Information: transmembrane anterior posterior transformation protein 1 homolog; cytomegalovirus partial fusion receptor
• UniProt Protein Name: Transmembrane anterior posterior transformation protein 1 homolog
• Protein Family: Transmembrane anterior posterior transformation protein
• UniProt Gene Name: TAPT1
• UniProt Synonym Gene Names: CMVFR
• UniProt Entry Name: TAPT1_HUMAN

NCBI Description

This gene encodes a highly conserved, putative transmembrane protein. A mutation in the mouse ortholog of this gene results in homeotic, posterior-to-anterior transformations of the axial skeleton which are similar to the phenotype of mouse homeobox C8 gene mutants. This gene is proposed to function downstream of homeobox C8 to transduce extracellular patterning information during axial skeleton development. An alternatively spliced transcript variant encoding a substantially different isoform has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TAPT1: May act as a downstream effector of HOXC8 possibly by transducing or transmitting extracellular information required for axial skeletal patterning during development. In case of infection, may act as a fusion receptor for cytomegalovirus (HCMV) strain AD169. Belongs to the TAPT1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 4p15.32

Cellular Component: integral to membrane

Molecular Function: growth hormone-releasing hormone receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; in utero embryonic development; embryonic skeletal development; post-embryonic development