Western Blot (WB)
(HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4 degree C. Detected by chemiluminescence.)
Goat TANK2 Polyclonal Antibody | anti-TNKS2 antibody
Goat anti-TANK2 Antibody
Western Blot (WB)
(HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4 degree C. Detected by chemiluminescence.)
Western Blot (WB)
(HEK293 overexpressing TANK2 (lane 4) and TANK1 (lane 2) and probed (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4 degree C. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
Uniprot Description
TNKS2: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP- ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates poly-ADP-ribosylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates poly-ADP-ribosylation of TERF1, thereby contributing to the regulation of telomere length. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles.
Protein type: Nuclear envelope; Transferase; EC 2.4.2.30
Chromosomal Location of Human Ortholog: 10q23.3
Cellular Component: cytoplasm; cytosol; Golgi membrane; nuclear chromosome, telomeric region; nuclear envelope; nucleus; pericentriolar material; perinuclear region of cytoplasm
Molecular Function: enzyme binding; metal ion binding; NAD+ ADP-ribosyltransferase activity; protein binding
Biological Process: multicellular organism growth; positive regulation of telomere maintenance via telomerase; protein amino acid ADP-ribosylation; protein polyubiquitination; regulation of multicellular organism growth; Wnt receptor signaling pathway
Research Articles on TNKS2
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Product Notes
The TNKS2 tnks2 (Catalog #AAA421677) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-TANK2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's TANK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB-Trf). Western Blot: In transfected HEK293 transiently expressing TANK2 a band of approx.127kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 127 kDa according to NP_079511.1. Recommended concentration, 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the TNKS2 tnks2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TANK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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