Rabbit anti-Human TANK Polyclonal Antibody | anti-TANK antibody

RABBIT ANTI TANK

Cat. Num. AAA224846

• Gene Names: TANK; ITRAF; TRAF2; I-TRAF
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: TANK; Polyclonal Antibody; RABBIT ANTI TANK; anti-TANK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TANK
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.2 mg/ml (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-TANK antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: Immunoprecipitation: 10; 20 ug/ml; Immunohistochemistry-Paraffin: 10; 20 ug/ml; Western Blot: 0.5; 4.0 ug/ml
• Reactivity Notes: Mouse, Rat
• Preservative Stabilisers: 30% Glycerol, 0.5% Bovine Serum Albumin, 0.01% Thiomersal
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: Store at -20 degree C Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. ; Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of mouse small intestine tissue lysate probed with Rabbit anti TANK antibody)

Related Product Information for anti-TANK antibody

Rabbit anti TANK antibody recognizes the TRAF family member-associated NF-kappa-B activator. The tumor necrosis factor receptor-associated factor (TRAF) family of proteins bind with and transduce signals from members of the tumor necrosis factor receptor superfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 143811466
• NCBI GeneID: 10010
• UniProt Accession #: Q92844; Q7Z4J6; Q92885; D3DPB5
• Molecular Weight: 13,626 Da
• NCBI Official Full Name: TRAF family member-associated NF-kappa-B activator
• NCBI Official Synonym Full Names: TRAF family member associated NFKB activator
• NCBI Official Symbol: TANK
• NCBI Official Synonym Symbols: ITRAF; TRAF2; I-TRAF
• NCBI Protein Information: TRAF family member-associated NF-kappa-B activator
• UniProt Protein Name: TRAF family member-associated NF-kappa-B activator
• Protein Family: Tankyrase
• UniProt Gene Name: TANK
• UniProt Synonym Gene Names: ITRAF; TRAF2; I-TRAF
• UniProt Entry Name: TANK_HUMAN

NCBI Description

The TRAF (tumor necrosis factor receptor-associated factor) family of proteins associate with and transduce signals from members of the tumor necrosis factor receptor superfamily. The protein encoded by this gene is found in the cytoplasm and can bind to TRAF1, TRAF2, or TRAF3, thereby inhibiting TRAF function by sequestering the TRAFs in a latent state in the cytoplasm. For example, the protein encoded by this gene can block TRAF2 binding to LMP1, the Epstein-Barr virus transforming protein, and inhibit LMP1-mediated NF-kappa-B activation. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

TANK: Acts as a regulator of TRAF function by maintaining them in a latent state. Overexpression inhibits TRAF2-mediated NF- kappa-B activation signaled by CD40, TNFR1 and TNFR2. Blocks TRAF2 binding to LMP1 and inhibits LMP1-mediated NF-kappa-B activation. May be involved in I-kappa-B-kinase (IKK) regulation; may function as an adapter for kinases such as TBK1 or IKBKE that can modulate IKK activity. Interacts with TBK1 (via TRAF-C domain). Interacts with TRAF1 (via TRAF-C domain). Interacts with TRAF2 (via TRAF-C domain); the interaction is disrupted by the phosphorylation of TANK by IKBKE. Interacts with TRAF3 (via TRAF-C domain); the interaction with TRAF3 is weaker than the interactions with TRAF1 and TRAF3. Interacts with IKBKG; the interaction is enhanced by IKBKE and TBK1. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 2q24.2

Cellular Component: cytoplasm; cytosol

Molecular Function: metal ion binding; protein binding; ubiquitin protein ligase binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; innate immune response; MyD88-independent toll-like receptor signaling pathway; signal transduction; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway