Rabbit TAL1 Polyclonal Antibody | anti-TAL1 antibody

TAL1 (Acetyl-K221/K222) Polyclonal Antibody

Cat. Num. AAA3007891

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TAL1; Polyclonal Antibody; TAL1 (Acetyl-K221/K222) Polyclonal Antibody; T-cell acute lymphocytic leukemia protein 1; TAL-1; Class A basic helix-loop-helix protein 17; bHLHa17; Stem cell protein; T-cell leukemia/lymphoma protein 5; T-cell acute lymphocytic leukemia protein 2; TAL-2; Class A basic helix-loop-helix protein 19; bHLHa19; TAL2; anti-TAL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TAL1 (Acetyl-K221/K222) polyclonal antibody detects endogenous levels of TAL1 protein only when acetylated at Lys221 and Lys222. This antibody also recognizes TAL2 protein when acetylated at Lys36 and Lys37.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 331

• Applicable Applications for anti-TAL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic Acetylated peptide derived from human TAL1 around the Acetylation site of K221/K222.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TAL1 (Acetyl-K221/K222) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) ) Lane3:PC12 whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Related Product Information for anti-TAL1 antibody

Activation of TAL1 characterizes up to 60% ofcases of human T cell acute lymph oblastic leukemia, making it themost frequent gain-of-function mutation observed in this disorder. TAL1 (also designated SCL) is a serine phospho protein and basic helixx-loop-helixtranscription factorknown to regulate embryonic hematopoiesis. This transcription factor binds as a heterodimer with E2A and HEB/HTF4 to a nucleotide sequence motif termed the E-box.In addition, leukemogenesisis accelerated dramatically bytransgenic coexpression of TAL1 and the catalyticsubunit ofcasein kinase IIalpha, a serine/threonine protein kinase known tomodulate the activity of other betaHLH transcription factors.

NCBI and Uniprot Product Information

• NCBI GI #: 4507363
• NCBI Accession #: NP_003180.1
• NCBI GenBank Nucleotide #: NP_003180.1
• UniProt Accession #: P17542; Q16559
• Molecular Weight: ~ 34kDa
• NCBI Official Full Name: T-cell acute lymphocytic leukemia protein 1 isoform 1
• UniProt Protein Name: T-cell acute lymphocytic leukemia protein 1
• Protein Family: Transaldolase
• UniProt Gene Name: TAL1
• UniProt Synonym Gene Names: BHLHA17; SCL; TCL5; TAL-1; bHLHa17
• UniProt Entry Name: TAL1_HUMAN

Uniprot Description

TAL1: a basic helix-loop-helix transcription. Regulates differentiation and survival during hemopoiesis. Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of eryhtroid differentiation. Mutations are associated with T-cell leukemia and melanoma. Binds to the LIM domain containing protein Rhombotin-2.

Protein type: DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: transcription factor complex; histone deacetylase complex; nuclear chromatin; nucleus

Molecular Function: protein binding; enzyme binding; protein heterodimerization activity; histone deacetylase binding; chromatin binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; erythrocyte maturation; cell fate commitment; positive regulation of chromatin assembly or disassembly; embryonic hemopoiesis; megakaryocyte differentiation; positive regulation of transcription, DNA-dependent; positive regulation of erythrocyte differentiation; astrocyte fate commitment; locomotory behavior; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; positive regulation of protein complex assembly; positive regulation of cell division; erythrocyte differentiation; spinal cord association neuron differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; platelet formation; basophil differentiation

Disease: Leukemia, Acute Lymphoblastic