Rabbit TAK1 Polyclonal Antibody | anti-TAK1 antibody

TAK1 (Phospho-Thr184) Antibody

Cat. Num. AAA9400910

• Gene Names: MAP3K7; TAK1; MEKK7; TGF1a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: TAK1; Polyclonal Antibody; TAK1 (Phospho-Thr184) Antibody; EC 2.7.11.25; M3K7; MAP3K7; Mitogen-activated protein kinase kinase kinase 7; TGF-beta-activated kinase 1; Transforming growth factor-beta-activated kinase 1; kinase TAK1; anti-TAK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 579

• Applicable Applications for anti-TAK1 antibody: Western Blot (WB)
• Application Notes: Western blotting: 1:500~1:3000
• Immunogen Type: peptide
• Immunogen Description: Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1.
• Modification: Phospho-Thr184
• Target Name: TAK1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody. The lane on the right is treated with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of extracts from 293 cells (Lane 2), using TAK1 (Phospho-Thr184) Antibody. The lane on the left is treated with synthesized peptide.)

Related Product Information for anti-TAK1 antibody

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7.

Product Categories/Family for anti-TAK1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4507361
• NCBI GeneID: 6885
• NCBI Accession #: NP_003179.1
• NCBI GenBank Nucleotide #: NM_003188.3
• UniProt Accession #: O43318; O43317; O43319; Q5TDN2; Q5TDN3; Q5TDT7; Q9NTR3; Q9NZ70; B2RE27; E1P523
• Molecular Weight: 53,740 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1
• UniProt Entry Name: M3K7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.25; Protein kinase, TKL; Kinase, protein; TKL group; MLK family; TAK1 subfamily

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; endosome membrane; IkappaB kinase complex; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of JNK activity; activation of MAPKK activity; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; positive regulation of T cell cytokine production; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; transforming growth factor beta receptor signaling pathway; JNK cascade; angiogenesis; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor signaling pathway; positive regulation of interleukin-2 production; innate immune response; toll-like receptor 9 signaling pathway; positive regulation of T cell activation; neural tube formation; I-kappaB phosphorylation