Rabbit TAK1 Polyclonal Antibody | anti-MAP3K7 antibody

Anti-TAK1 Antibody

Cat. Num. AAA178506

• Gene Names: MAP3K7; TAK1; MEKK7; TGF1a
• Reactivity: Human, Rat; No cross reactivity with other proteins.
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: TAK1; Polyclonal Antibody; Anti-TAK1 Antibody; MAP3K 7; MAP3K7; MEKK7; TGF1a; O43318; Mitogen-activated protein kinase kinase kinase 7; anti-MAP3K7 antibody

• Host: Rabbit
• Reactivity: Human, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 579

• Applicable Applications for anti-MAP3K7 antibody: Western Blot (WB)
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Human, Rat; Tested Species:In-house tested species with positive results.; Other applications have not been tested.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TAK1 (566-603aa RLVQEHKKLLDENKSLSTYYQQCKKQLEVIRSQQQKRQ), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of TAK1 expression in rat spleen extract (lane 1) and HELA whole cell lysates (lane 2). TAK1 at 79KD was detected using rabbit anti- TAK1 Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-MAP3K7 antibody

Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 7 (MAP3K7) detection.
Background: Mitogen-activated protein kinase kinase kinase 7, also known as TAK1, is an enzyme that in humans is encoded by the MAP3K7 gene. The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

References

1. "Entrez Gene: MAP3K7 mitogen-activated protein kinase kinase kinase 7".
2. Kondo M, Osada H, Uchida K, Yanagisawa K, Masuda A, Takagi K, Takahashi T, Takahashi T (February 1998). "Molecular cloning of human TAK1 and its mutational analysis in human lung cancer". Int J Cancer 75 (4): 559-63.
3. Ninomiya-Tsuji J, Kishimoto K, Hiyama A, Inoue J, Cao Z, Matsumoto K (March 1999). "The kinase TAK1 can activate the NIK-I kappaB as well as the MAP kinase cascade in the IL-1 signalling pathway".Nature 398 (6724): 252-6.

NCBI and Uniprot Product Information

• NCBI GI #: 4507361
• NCBI GeneID: 6885
• NCBI Accession #: NP_003179.1
• NCBI GenBank Nucleotide #: NM_003188.3
• UniProt Accession #: O43318; O43317; O43319; Q5TDN2; Q5TDN3; Q5TDT7; Q9NTR3; Q9NZ70; B2RE27; E1P523
• Molecular Weight: 53,740 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: MAP3K7
• NCBI Official Synonym Symbols: TAK1; MEKK7; TGF1a
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: Mitogen-activated protein kinase kinase kinase
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1
• UniProt Entry Name: M3K7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; TAK1 subfamily

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: cytoplasm; cytosol; endosome membrane; IkappaB kinase complex; nucleus

Molecular Function: MAP kinase kinase kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; I-kappaB kinase/NF-kappaB cascade; I-kappaB phosphorylation; JNK cascade; MyD88-dependent toll-like receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 production; positive regulation of JNK activity; positive regulation of T cell activation; positive regulation of T cell cytokine production; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; transforming growth factor beta receptor signaling pathway; Wnt receptor signaling pathway, calcium modulating pathway