Rabbit anti-Human, Mouse TAF1A Polyclonal Antibody | anti-TAF1A antibody

TAF1A (TATA Box-binding Protein-associated Factor RNA Polymerase I Subunit A, RNA Polymerase I-specific TBP-associated Factor 48kD, TATA Box-binding Protein-associated Factor 1A, Transcription Factor SL1, Transcription Initiation Factor SL1/TIF-IB Subunit

Cat. Num. AAA6395895

• Gene Names: TAF1A; SL1; RAFI48; TAFI48; MGC:17061
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF1A; Polyclonal Antibody; TAF1A (TATA Box-binding Protein-associated Factor RNA Polymerase I Subunit A; RNA Polymerase I-specific TBP-associated Factor 48kD; TATA Box-binding Protein-associated Factor 1A; Transcription Factor SL1; Transcription Initiation Factor SL1/TIF-IB Subunit; anti-TAF1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TAF1A. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 450

• Applicable Applications for anti-TAF1A antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TAF1A, aa1-225 (NP_005672.1).
• Immunogen Sequence: MDLEESEEFKERCTQCAAVSWGLTDEGKYYCTSCHNVTERYQEVTNTDLIPNTQIKALNRGLKKKNNTEKGWDWYVCEGFQYILYQQAEALKNLGVGPELKNDVLHNFWKRYLQKSKQAYCKNPVYTTGRKPTVLEDNLSHSDWASEPELLSDVSCPPFLESGAESQSDIHTRKPFPVSKASQSETSVCSGSLDGVEYSQRKEKGIVKMTMPQTLAFCYLSLLWQREAITLSDLLRFVEEDHIPYINAFQHFPEQMKLYGRDRGIFGIESWPDYEDIYKKTIEVGTFLDLPRFPDITEDCYLHPNILCMKYLMEVNLPDEMHSLTCHVVKMTGMGEVDFLTFDPIAKMAKAVKYDVQAVAIIVVVLKLLFLLDDSFEWSLSNLAEKHNEKNKKDKPWFDFRKWYQIMKKAFDEKKQKWEEARAKYLWKSEKPLYYSFVDKPVAYKKREMVVNLQKQFSTLVDSTATAGKKSPSSFQFNWTEEDTDRTCFHGHSLQGVLKEKGQSLLTKNSLYWLSTQKFCRCYCTHVTTYEESNYSLSYQFILNLFSFLLRIKTSLLHEEVSLVEKKLFEKKYSVKRKKSRSKKVRRH
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TAF1B expression in transfected 293T cell line by TAF1B polyclonal antibody. Lane 1: TAF1B transfected lysate (68.8kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TAF1A antibody

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Product Categories/Family for anti-TAF1A antibody

Antibodies; Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 5032143
• NCBI GeneID: 9015
• NCBI Accession #: NP_005672.1
• NCBI GenBank Nucleotide #: NM_005681.2
• UniProt Accession #: Q15573
• NCBI Official Full Name: TATA box-binding protein-associated factor RNA polymerase I subunit A isoform 1
• NCBI Official Synonym Full Names: TATA-box binding protein associated factor, RNA polymerase I subunit A
• NCBI Official Symbol: TAF1A
• NCBI Official Synonym Symbols: SL1; RAFI48; TAFI48; MGC:17061
• NCBI Protein Information: TATA box-binding protein-associated factor RNA polymerase I subunit A
• UniProt Protein Name: TATA box-binding protein-associated factor RNA polymerase I subunit A
• Protein Family: TATA box-binding protein-associated factor
• UniProt Gene Name: TAF1A
• UniProt Synonym Gene Names: TAFI48
• UniProt Entry Name: TAF1A_HUMAN

NCBI Description

This gene encodes a subunit of the RNA polymerase I complex, Selectivity Factor I (SLI). The encoded protein is a TATA box-binding protein-associated factor that plays a role in the assembly of the RNA polymerase I preinitiation complex. Alternate splicing results in multiple transcript variants encoding multiple isoforms.[provided by RefSeq, Jan 2011]

Uniprot Description

TAF1A: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: microtubule cytoskeleton; nucleoplasm; intracellular membrane-bound organelle; RNA polymerase I transcription factor complex; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; chromatin silencing at rDNA; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic