Rabbit anti-Human TAB1 Polyclonal Antibody | anti-TAB1 antibody

TAB1, Phosphorylated (Ser423) (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, 3'-Tab1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, MAP3K7IP1) (Bi

Cat. Num. AAA6501933

• Gene Names: TAB1; 3'-Tab1; MAP3K7IP1
• Reactivity: Human
• Applications: ELISA, Dot Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: TAB1; Polyclonal Antibody; Phosphorylated (Ser423) (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1; TGF-beta-activated Kinase 1-binding Protein 1; TAK1-binding Protein 1; 3'-Tab1; Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1; MAP3K7IP1) (Bi; TGF-beta Activated Kinase 1/MAP3K7 Binding Protein 1; MGC57664; anti-TAB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP3K7IP1 when phosphorylated at Ser423.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TAB1 antibody: ELISA (EIA), Dot Blot (DB)
• Application Notes: ELISA: 1:1,000; Dot Blot: 1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic phosphopeptide corresponding to the C-terminal of human MAP3K7IP1 (KLH).
• Conjugate: Biotin
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-MAP3K7IP1-pS423 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Product Categories/Family for anti-TAB1 antibody

Antibodies; Binding Proteins

References

1. Conner, S.H., Biochem. J. 399(3), 427-434 (2006). 2. Zhou, H., Mol. Cell. Biol. 26(10), 3824-3834 (2006). 3. Singhirunnusorn, P., J. Biol. Chem. 280(8), 7359-7368 (2005). 4. Jin, J., Curr. Biol. 14(16), 1436-1450 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 5174703
• NCBI GeneID: 10454
• NCBI Accession #: NP_006107
• NCBI GenBank Nucleotide #: NM_006116.2
• UniProt Accession #: Q15750; Q2PP09; Q8IZW2
• Molecular Weight: 49,917 Da
• NCBI Official Full Name: TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 isoform alpha
• NCBI Official Synonym Full Names: TGF-beta activated kinase 1/MAP3K7 binding protein 1
• NCBI Official Symbol: TAB1
• NCBI Official Synonym Symbols: 3'-Tab1; MAP3K7IP1
• NCBI Protein Information: TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TAK1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1; mitogen-activated protein kinase kinase kinase 7-interacting protein 1
• UniProt Protein Name: TGF-beta-activated kinase 1 and MAP3K7-binding protein 1
• Protein Family: TGF-beta-activated kinase 1 and MAP3K7-binding protein
• UniProt Gene Name: TAB1
• UniProt Synonym Gene Names: MAP3K7IP1; TAK1-binding protein 1
• UniProt Entry Name: TAB1_HUMAN

NCBI Description

The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

TAB1: May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis. Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Activator

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; protein complex; cytoplasm; endosome membrane; cytosol

Molecular Function: protein binding; mitogen-activated protein kinase p38 binding; enzyme activator activity; kinase activator activity; protein complex binding; catalytic activity

Biological Process: heart morphogenesis; I-kappaB kinase/NF-kappaB cascade; in utero embryonic development; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; activation of MAPKKK activity; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; lung development