Rabbit anti-Human T-Box Protein 3 (TBX3) Polyclonal Antibody | anti-TBX3 antibody

Biotin-Linked Antibody to T-Box Protein 3 (TBX3)

Cat. Num. AAA2004092

• Gene Names: TBX3; UMS; XHL; TBX3-ISO
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: T-Box Protein 3 (TBX3); Polyclonal Antibody; Biotin-Linked Antibody to T-Box Protein 3 (TBX3); anti-TBX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against TBX3 conjugated to biotin. It has been selected for its ability to recognize TBX3 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/ml (varies by lot)
• Sequence Length: 723

• Applicable Applications for anti-TBX3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Knockout Validation

(Knockout Validation: Lane 1: Wild-type HepG2 cell lysate; Lane 2: TBX3 knockout HepG2 cell lysate; Predicted MW: 79,77,65kd Observed MW: 70kd Primary Ab: 2ug/ml Rabbit Anti-Human TBX3 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))

Product Categories/Family for anti-TBX3 antibody

Biotin Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 47419905
• NCBI GeneID: 6926
• NCBI Accession #: NP_005987.3
• NCBI GenBank Nucleotide #: NM_005996.3
• UniProt Accession #: O15119; Q8TB20; Q9UKF8
• Molecular Weight: 79,389 Da
• NCBI Official Full Name: T-box transcription factor TBX3 isoform 1
• NCBI Official Synonym Full Names: T-box 3
• NCBI Official Symbol: TBX3
• NCBI Official Synonym Symbols: UMS; XHL; TBX3-ISO
• NCBI Protein Information: T-box transcription factor TBX3; T-box protein 3; bladder cancer related protein XHL
• UniProt Protein Name: T-box transcription factor TBX3
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX3
• UniProt Synonym Gene Names: T-box protein 3
• UniProt Entry Name: TBX3_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TBX3: Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation. Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS). UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12q24.21

Cellular Component: nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: embryonic forelimb morphogenesis; positive regulation of transcription, DNA-dependent; limbic system development; negative regulation of epithelial cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; female genitalia development; forelimb morphogenesis; mammary gland development; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; male genitalia development; heart looping; skeletal development; luteinizing hormone secretion; specification of organ position; blood vessel development; transcription, DNA-dependent; in utero embryonic development; stem cell maintenance; cell aging; positive regulation of cell cycle; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; mesoderm morphogenesis; embryonic digit morphogenesis; follicle-stimulating hormone secretion; negative regulation of myoblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Ulnar-mammary Syndrome