Rabbit anti-Human SYT5 Polyclonal Antibody | anti-SYT5 antibody

SYT5, ID (SYT5, Synaptotagmin-5, Synaptotagmin V) (MaxLight 550)

Cat. Num. AAA6353035

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYT5; Polyclonal Antibody; ID (SYT5; Synaptotagmin-5; Synaptotagmin V) (MaxLight 550); Synaptotagmin V; anti-SYT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.

• Applicable Applications for anti-SYT5 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SYT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-167 amino acids from the Central region of human SYT5.
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-SYT5 antibody

SYT5 may be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)-dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface (By similarity).

Product Categories/Family for anti-SYT5 antibody

Antibodies; Syntaxin; Synaptotagmin; Synaptophysin

References

Martins-de-Souza, D., et al. J Psychiatr Res (2010). In press: Sreenath, A.S., et al. Clin. Biochem. 38(5):436-443(2005). Mizutani, A., et al. J. Biol. Chem. 275(13):9823-9831(2000). Craxton, M., et al. FEBS Lett. 460(3):417-422(1999). Fukuda, M., et al. J. Biol. Chem. 274(44):31421-31427(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 92859638
• NCBI GeneID: 6861
• NCBI Accession #: NP_003171.2
• NCBI GenBank Nucleotide #: NM_003180.2
• UniProt Accession #: O00445; Q86X72; B3KWJ8
• Molecular Weight: 42,900 Da
• NCBI Official Full Name: synaptotagmin-5
• NCBI Official Synonym Full Names: synaptotagmin V
• NCBI Official Symbol: SYT5
• NCBI Protein Information: synaptotagmin-5; sytV; synaptotagmin 5
• UniProt Protein Name: Synaptotagmin-5
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT5
• UniProt Synonym Gene Names: SytV
• UniProt Entry Name: SYT5_HUMAN

NCBI Description

Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]

Uniprot Description

SYT5: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Belongs to the synaptotagmin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.42|11p

Cellular Component: neuron projection; cell soma; synaptic vesicle membrane; recycling endosome membrane; perinuclear region of cytoplasm; integral to membrane; cell junction

Molecular Function: clathrin binding; syntaxin binding; calcium-dependent phospholipid binding; protein heterodimerization activity; transporter activity; metal ion binding

Biological Process: synaptic transmission; energy reserve metabolic process; regulation of insulin secretion; calcium ion-dependent exocytosis