Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (All lanes : Anti-SYP Antibody (C-term) at 1:8000 dilutionLane 1: human brain lysateLane 2: human cerebellum lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Rabbit SYP Polyclonal Antibody | anti-SYP antibody

SYP Antibody (C-term)

Gene Names
SYP; MRX96; MRXSYP
Reactivity
Human, Mouse; Predicted Reactivity: Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
SYP; Polyclonal Antibody; SYP Antibody (C-term); Synaptophysin; Major synaptic vesicle protein p38; anti-SYP antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse; Predicted Reactivity: Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This SYP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from the C-terminal region of human SYP.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
313
Applicable Applications for anti-SYP antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
Application Notes
WB:~~1:1000
IHC-P:~~1:10~50
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
Tissue Location
Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
Antigen Region
225-253 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-SYP Antibody (C-term) at 1:8000 dilutionLane 1: human brain lysateLane 2: human cerebellum lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (All lanes : Anti-SYP Antibody (C-term) at 1:8000 dilutionLane 1: human brain lysateLane 2: human cerebellum lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western blot analysis of SYP (arrow) using rabbit polyclonal SYP Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SYP gene.)

Western Blot (WB) (Western blot analysis of SYP (arrow) using rabbit polyclonal SYP Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SYP gene.)

Western Blot (WB)

(SYP Antibody (C-term) western blot analysis in mouse brain,cerebellum tissue lysates (35ug/lane).This demonstrates the SYP antibody detected the SYP protein (arrow).)

Western Blot (WB) (SYP Antibody (C-term) western blot analysis in mouse brain,cerebellum tissue lysates (35ug/lane).This demonstrates the SYP antibody detected the SYP protein (arrow).)

Immunohistochemistry (IHC)-Paraffin

(SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)-Paraffin (SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)-Paraffin

(SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC)-Paraffin (SYP Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SYP Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-SYP antibody
Description: Synaptophysin (p38) is an integral membrane protein of small synaptic vesicles in brain and endocrine cells. [supplied by OMIM].

Background: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity).
Product Categories/Family for anti-SYP antibody
References
Ishida, M., et al. Oncol. Rep. 22 (4):733-737 (2009). Sterlacci, W., et al. Virchows Arch. 455 (2):125-132 (2009). Head, E., et al. Neurobiol. Aging 30 (7):1125-1134 (2009). Gulubova, M., et al. J. Gastroenterol. Hepatol. 23 (10):1574-1585 (2008). Glantz, L.A., et al. Neuroscience 149 (3):582-591 (2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33845 Da
NCBI Official Full Name
synaptophysin
NCBI Official Synonym Full Names
synaptophysin
NCBI Official Symbol
SYP
NCBI Official Synonym Symbols
MRX96; MRXSYP
NCBI Protein Information
synaptophysin
UniProt Protein Name
Synaptophysin
Protein Family
UniProt Gene Name
SYP
UniProt Entry Name
SYPH_HUMAN

NCBI Description

This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]

Uniprot Description

SYP: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity. Homohexamer or homotetramer. Interacts with SRCIN1. Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype. Belongs to the synaptophysin/synaptobrevin family.

Protein type: Membrane protein, multi-pass; Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp11.23-p11.22

Cellular Component: cell junction; excitatory synapse; integral to synaptic vesicle membrane; neuron projection; presynaptic active zone; presynaptic membrane; synaptic vesicle; synaptic vesicle membrane; terminal button

Molecular Function: cholesterol binding; identical protein binding; protein self-association; SH2 domain binding; transporter activity

Biological Process: endocytosis; regulation of long-term neuronal synaptic plasticity; regulation of neuronal synaptic plasticity; regulation of short-term neuronal synaptic plasticity; synaptic vesicle maturation; synaptic vesicle membrane organization and biogenesis

Disease: Mental Retardation, X-linked 96

Research Articles on SYP

Similar Products

Product Notes

The SYP syp (Catalog #AAA9217083) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SYP Antibody (C-term) reacts with Human, Mouse; Predicted Reactivity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SYP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA). WB:~~1:1000 IHC-P:~~1:10~50. Researchers should empirically determine the suitability of the SYP syp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.