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Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: PC-12) using a 12 % SDS PAGE gel and Syntaxin 5 antibody at a dilution of 1:5000)

Rabbit Syntaxin 5 Polyclonal Antibody | anti-STX5 antibody

Syntaxin 5 antibody

Gene Names
STX5; SED5; STX5A
Reactivity
Xenopus laevis, Pig, Rhesus Monkey, Bovine.
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Syntaxin 5 antibody was purified by antigen-affinity chromatography
Synonyms
Syntaxin 5; Polyclonal Antibody; Syntaxin 5 antibody; Polyclonal Syntaxin 5; Anti-Syntaxin 5; STX5A; Syntaxin -5; syntaxin-5; syntaxin 5; SED5; syntaxin 5A; STX5; anti-STX5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Xenopus laevis, Pig, Rhesus Monkey, Bovine.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Syntaxin 5 antibody was purified by antigen-affinity chromatography
Form/Format
Affinity purified liquid in 100mM Tris, pH 7.0, with 100 mM Glycine, 20% Glycerol, and 0.01% Thimerosal.
Concentration
1.01 mg/ml (varies by lot)
Sequence Length
301
Applicable Applications for anti-STX5 antibody
Suitable for use in ICC/IF, IHC, WB.
Application Notes
ICC/IFF (1:100-1:1000)
IHC (1:100-1,000)
WB (1:100-1:10,000)
Immunogen
Recombinant protein encompassing a propietary sequence within the center region of human Syntaxin 5.
Biological Significance
Syntaxin 5 mediates endoplasmic reticulum to Golgi transport.
Cross-Reactivity
Human
Preparation and Storage
Store at 4°C for short term, -20°C for long term. Avoid repeat freeze-thaws.

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: PC-12) using a 12 % SDS PAGE gel and Syntaxin 5 antibody at a dilution of 1:5000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: PC-12) using a 12 % SDS PAGE gel and Syntaxin 5 antibody at a dilution of 1:5000)

Western Blot (WB)

(Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 12 % SDS PAGE gel and Syntaxin 5 antibody at a dilution of 1:5000)

Western Blot (WB) (Western blot analysis of 30 ug of whole cell lysate (A: A431) using a 12 % SDS PAGE gel and Syntaxin 5 antibody at a dilution of 1:5000)

Immunofluorescence (IF)

(Immunofluorescence analysis of methanol-fixed A431, using Syntaxin 5 antibody at 1:200 dilution.)

Immunofluorescence (IF) (Immunofluorescence analysis of methanol-fixed A431, using Syntaxin 5 antibody at 1:200 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of paraffin-embedded MCF7 xenograft using Syntaxin 5 antibody at a dilution of 1:500)

Immunohistochemistry (IHC) (Immunohistochemical staining of paraffin-embedded MCF7 xenograft using Syntaxin 5 antibody at a dilution of 1:500)
Related Product Information for anti-STX5 antibody
Affinity purified Rabbit polyclonal Syntaxin 5 antibody
Product Categories/Family for anti-STX5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
30 kDa
NCBI Official Full Name
syntaxin 5
NCBI Official Synonym Full Names
syntaxin 5
NCBI Official Symbol
STX5
NCBI Official Synonym Symbols
SED5; STX5A
NCBI Protein Information
syntaxin-5
UniProt Protein Name
Syntaxin-5
Protein Family
UniProt Gene Name
STX5
UniProt Synonym Gene Names
STX5A
UniProt Entry Name
STX5_HUMAN

NCBI Description

This gene encodes a member of the syntaxin or t-SNARE (target-SNAP receptor) family. These proteins are found on cell membranes and serve as the targets for v-SNAREs (vesicle-SNAP receptors), permitting specific synaptic vesicle docking and fusion. The encoded protein regulates endoplasmic reticulum to Golgi transport and plays a critical role in autophagy. Autoantibodies targeting the encoded protein may be a diagnostic marker for endometriosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

STX5: Mediates endoplasmic reticulum to Golgi transport. Belongs to the syntaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: nucleoplasm; Golgi membrane; SNARE complex; Golgi apparatus; ER-Golgi intermediate compartment membrane; endoplasmic reticulum; integral to membrane; vesicle

Molecular Function: SNAP receptor activity; SNARE binding; protein binding; protein N-terminus binding

Biological Process: vesicle fusion; intracellular protein transport; ER to Golgi vesicle-mediated transport; vesicle docking; positive regulation of protein catabolic process; vesicle fusion with Golgi apparatus; retrograde transport, endosome to Golgi

Research Articles on STX5

Similar Products

Product Notes

The STX5 stx5 (Catalog #AAA835354) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Syntaxin 5 antibody reacts with Xenopus laevis, Pig, Rhesus Monkey, Bovine. and may cross-react with other species as described in the data sheet. AAA Biotech's Syntaxin 5 can be used in a range of immunoassay formats including, but not limited to, Suitable for use in ICC/IF, IHC, WB. ICC/IFF (1:100-1:1000) IHC (1:100-1,000) WB (1:100-1:10,000). Researchers should empirically determine the suitability of the STX5 stx5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Syntaxin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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