Rabbit anti-Human SYNCI Polyclonal Antibody | anti-SYNCI antibody

SYNCI, NT (SYNC, SYNC1, Syncoilin, Syncoilin intermediate filament 1, Syncoilin-1) (FITC)

Cat. Num. AAA6352866

• Gene Names: SYNC; SYNC1; SYNCOILIN
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SYNCI; Polyclonal Antibody; NT (SYNC; SYNC1; Syncoilin; Syncoilin intermediate filament 1; Syncoilin-1) (FITC); SYNC; Syncoilin-1; anti-SYNCI antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 482

• Applicable Applications for anti-SYNCI antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SYNCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-163 amino acids from the N-terminal region of human SYNCI.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SYNCI Antibody (N-term) western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).This demonstrates the SYNCI antibody detected the SYNCI protein (arrow).)

Immunohistochemistry (IHC)

(SYNCI Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SYNCI Antibody (N-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-SYNCI antibody

This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SYNCI antibody

Antibodies; Cardiac Markers

References

Wakayama, Y., et al. Int. J. Neurosci. 120(2):144-149(2010). Jordanova, A., et al. Nat. Genet. 38(2):197-202(2006). Brown, S.C., et al. Muscle Nerve 32(6):715-725(2005). Poon, E., et al. J. Biol. Chem. 277(5):3433-3439(2002). Newey, S.E., et al. J. Biol. Chem. 276(9):6645-6655(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 166706887
• NCBI GeneID: 81493
• NCBI Accession #: NP_110413.2
• NCBI GenBank Nucleotide #: NP_110413.2
• UniProt Accession #: Q9H7C4
• NCBI Official Full Name: syncoilin isoform 1
• NCBI Official Synonym Full Names: syncoilin, intermediate filament protein
• NCBI Official Symbol: SYNC
• NCBI Official Synonym Symbols: SYNC1; SYNCOILIN
• NCBI Protein Information: syncoilin
• UniProt Protein Name: Syncoilin
• UniProt Gene Name: SYNC
• UniProt Synonym Gene Names: SYNC1

NCBI Description

This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

Atypical type III intermediate filament (IF) protein that may play a supportive role in the efficient coupling of mechanical stress between the myofibril and fiber exterior. May facilitate lateral force transmission during skeletal muscle contraction. Does not form homofilaments nor heterofilaments with other IF proteins.