Western Blot (WB)
(Anti-SYNC rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:55 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human SYNC Polyclonal Antibody | anti-SYNC antibody
Anti-SYNC Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SYNC; Polyclonal Antibody; Anti-SYNC Antibody; Rabbit Polyclonal; SYNC Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-SYNC1 Antibody; Anti-SYNCOILIN Antibody; anti-SYNC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SYNC
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-SYNC antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human SYNC fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human SYNC fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-SYNC rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:55 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-SYNC rabbit polyclonal antibody at 1:500 dilution Lane A: Hela Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:55 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.))
Related Product Information for anti-SYNC antibody
Syncoilin (SYNC) is a member of the intermediate filament protein family, highly expressed in skeletal and cardiac muscle. Syncoilin binds alpha-dystrobrevin, a component of the dystrophin associated protein complex (DAPC) located at the muscle cell membrane, and desmin, a muscle-specific intermediate filament protein, thus providing a link between the DAPC and the muscle intermediate filament network. Syncoilin is a 64-kDa intermediate filament protein expressed in skeletal muscle and enriched at the perinucleus, sarcolemma, and myotendinous and neuromuscular junctions. Due to its pattern of cellular localization and binding partners, syncoilin is an ideal candidate to be both an important structural component of myocytes and a potential mediator of inherited myopathies.
References
Zhang J, et al. (2008) Syncoilin is required for generating maximum isometric stress in skeletal muscle but dispensable for muscle cytoarchitecture. Am J Physiol Cell Physiol 294 (5): C1175-1182.Moorwood C (2008) Syncoilin, an intermediate filament-like protein linked to the dystrophin associated protein complex in skeletal muscle. Cell Mol Life Sci 65 (19): 2957-2963.McCullagh KJ, et al. (2008) Analysis of skeletal muscle function in the c57bl6/sv129 syncoilin knockout mouse. Mamm Genome 19 (5): 339-351.
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Product Notes
The SYNC (Catalog #AAA8109918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SYNC Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SYNC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SYNC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYNC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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