Rabbit anti-Human Synaptotagmin-1 Polyclonal Antibody | anti-SYT1 antibody

Anti-Human Synaptotagmin-1, Purified, (Polyclonal) (Rabbit IgG)

Cat. Num. AAA524119

• Gene Names: SYT1; P65; SYT; SVP65
• Reactivity: Human
• Applications: Western Blot
• Synonyms: Synaptotagmin-1; Polyclonal Antibody; Anti-Human Synaptotagmin-1; Purified; (Polyclonal) (Rabbit IgG); Purified Rabbit Anti-Human Synaptotagmin-1 Polyclonal Antibody; anti-SYT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Synaptotagmin-1 (This antibody is specific for human synaptotagmin-1)
• Form/Format: Purified
• Sequence Length: 422

• Applicable Applications for anti-SYT1 antibody: Western Blot (WB)
• Application Notes: Suggested minimum working dilution of 1:100 in Western Blot. This antibody has not been tested in other applications.
• Immunogen: 16 amino acid synthetic peptide of human synaptotagmin-1 (aa 108-122) conjugated to KLH.
• Presentation: 100 ug purified IgG buffered in 100 ul PBS and 0.02% NaN3 (1 mg/ml). (Purified from serum via affinity chromatography). For maximum recovery of contents, spin down tube before use.
• Preparation and Storage: For all formats, store at + 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Related Product Information for anti-SYT1 antibody

Synaptotagmin-1 is a synaptic vesicle membrane protein that belongs to the Synaptotagmins family; characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2-domains. Synaptotagmin-1, the first synaptotagmin studied, is an abundant synaptic vesicle protein that binds Ca2+ and phospholipids via both of its C2 domains and is essential for fast Ca2+- triggered neurotransmitter release. Multiple members (15) exist within the Synaptotagmins family with high homology in their C-terminal repeats and lowest in their N-terminal intravesicular sequences. The two internal repeats in synaptotagmin are homologous to the regulatory region of protein kinase C. Synaptotagmin -1 and 2 are highly homologous but differentially distributed, with Synaptotagmin-1 being highly expressed in the forebrain and some endocrine tissues and Synaptotagmin-2 in the brain stem and spinal cord.

NCBI and Uniprot Product Information

• NCBI GI #: 209447073
• NCBI GeneID: 6857
• NCBI Accession #: NP_001129278.1
• NCBI GenBank Nucleotide #: NM_001135806.1
• UniProt Accession #: P21579; Q6AI31
• Molecular Weight: 47,573 Da
• NCBI Official Full Name: synaptotagmin-1
• NCBI Official Synonym Full Names: synaptotagmin I
• NCBI Official Symbol: SYT1
• NCBI Official Synonym Symbols: P65; SYT; SVP65
• NCBI Protein Information: synaptotagmin-1; sytI; synaptotagmin 1
• UniProt Protein Name: Synaptotagmin-1
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT1
• UniProt Synonym Gene Names: SVP65; SYT; SytI
• UniProt Entry Name: SYT1_HUMAN

NCBI Description

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Uniprot Description

Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains

By similarity.

Subunit structure: Homotetramer

Probable. Interacts with SCAMP5, STON2, SV2A, SV2B, SV2C and RIMS1. Forms a complex with SV2B, syntaxin 1 and SNAP25

By similarity. Ref.6

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note: Synaptic vesicles and chromaffin granules.

Domain: The first C2 domain mediates Ca2+-dependent phospholipid binding.The second C2 domain mediates interaction with SV2A and STN2

By similarity.

Sequence similarities: Belongs to the synaptotagmin family.Contains 2 C2 domains.