Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SYCE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Human SYCE1 Polyclonal Antibody | anti-SYCE1 antibody
SYCE1 Polyclonal Antibody
Gene Names
SYCE1; CT76; C10orf94
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
SYCE1; Polyclonal Antibody; SYCE1 Polyclonal Antibody; CT76; C10orf94; anti-SYCE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
351
Applicable Applications for anti-SYCE1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
IHC: 1:50 - 1:200
IF: 1:50 - 1:100
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human SYCE1
Calculated Molecular Weight
40kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SYCE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using SYCE1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of MCF-7 cells using SYCE1 antibody. Blue: DAPI for nuclear staining.)
Product Categories/Family for anti-SYCE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
351
NCBI Official Full Name
Synaptonemal complex central element protein 1
NCBI Official Synonym Full Names
synaptonemal complex central element protein 1
NCBI Official Symbol
SYCE1
NCBI Official Synonym Symbols
CT76; C10orf94
NCBI Protein Information
synaptonemal complex central element protein 1; cancer/testis antigen 76
UniProt Protein Name
Synaptonemal complex central element protein 1
Protein Family
UniProt Gene Name
SYCE1
UniProt Synonym Gene Names
C10orf94; CT76
UniProt Entry Name
SYCE1_HUMAN
Uniprot Description
SYCE1: Major component of the transverse central element of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Requires SYCP1 in order to be incorporated into the central element. May have a role in the synaptonemal complex assembly, stabilization and recombination. Belongs to the SYCE family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 10q26.3
Cellular Component: central element
Biological Process: synaptonemal complex assembly; cell division
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Product Notes
The SYCE1 syce1 (Catalog #AAA129902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SYCE1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SYCE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the SYCE1 syce1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SYCE1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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