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Swine anti Rabbit Ig light chains kappa and lambda (free and bound) Secondary Antibody

Swine anti Rabbit Ig light chains kappa and lambda (free and bound)

Reactivity
Rabbit
Applications
Immunoprecipitation
Synonyms
Swine anti Rabbit Ig light chains kappa and lambda (free and bound); Polyclonal Secondary Antibody; Swine anti Rabbit Ig light chains kappa and lambda (free and bound) secondary antibody
Ordering
For Research Use Only!
Host
Swine
Reactivity
Rabbit
Clonality
Polyclonal
Specificity
Precipitating polyclonal Swine antiSerum to Rabbit light chains, free and bound
Form/Format
Delipidated, heat inactivated lyophilized stable whole serum.
Concentration
The protein and IgG concentration in the antiserum are comparable to those of pooled swine serum. No foreign proteins added. (varies by lot)
Applicable Applications for Swine anti Rabbit Ig light chains kappa and lambda (free and bound) secondary antibody
Immunoprecipitation (IP)
Application Notes
Immunoprecipitation. In immunoelectrophoresis use 2 1-11 or equivalent against 120 1-11 antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 101-11 antiserum in a 3 mm diameter centre well and 2 1-11 serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single immunodiffusion. use 1 % antiserum in agar gel to establish a stander curve. Preciptin titre not less than 1:64 when tested against normal rabbit serum in agar block titration.
Preservative
No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Immunogen
Purified light chains isolated from polyclonal rabbit IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.
Adsorption
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies reacting with the Fc fragments of immunoglobulins or with other plasma proteins.
Product Type
Secondary Antibodies.
Cross-reactivity Note
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, but a wide scale of reactivity with IgG of other mammalian species must be assumed. The degree of cross-reactivity depends in addition to the phylogenetic relationship also on the concentrations of the reactants and the sensitivity of the assay format.
Antibody Titre
Precipitin titre not less than 1:64 when tested against normal rabbit serum in agar block titration.
Preparation and Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at 4 degree C, prolonged storage at or below -20 degree C. It is reconstituted by adding 1ml sterile distilled water.

Lyophilized at 4 degree C: at least 10 years.
Reconstituted at or below -20 degree C: 3-5 years.
Reconstituted at 4 degree C: 7 days.
Product Categories/Family for Swine anti Rabbit Ig light chains kappa and lambda (free and bound) secondary antibody

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Product Notes

The Swine anti Rabbit Ig light chains kappa and lambda (free and bound) (Catalog #AAA571936) is a Secondary Antibody produced from Swine and is intended for research purposes only. The product is available for immediate purchase. The Swine anti Rabbit Ig light chains kappa and lambda (free and bound) reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's Swine anti Rabbit Ig light chains kappa and lambda (free and bound) can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Immunoprecipitation. In immunoelectrophoresis use 2 1-11 or equivalent against 120 1-11 antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 101-11 antiserum in a 3 mm diameter centre well and 2 1-11 serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. In single immunodiffusion. use 1 % antiserum in agar gel to establish a stander curve. Preciptin titre not less than 1:64 when tested against normal rabbit serum in agar block titration. Researchers should empirically determine the suitability of the Swine anti Rabbit Ig light chains kappa and lambda (free and bound) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Swine anti Rabbit Ig light chains kappa and lambda (free and bound), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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