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Western Blot (WB)
(Western Blot: Sample: Rat Lung Tissue.)
Rabbit anti-Rat Surfactant Associated Protein A Polyclonal Antibody | anti-SPA antibody
Polyclonal Antibody to Surfactant Associated Protein A (SPA)
Gene Names
Sftpa1; PSAP; SP-A; PSP-A; Sftp1; Sftpa; Sftpl
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Surfactant Associated Protein A; Polyclonal Antibody; Polyclonal Antibody to Surfactant Associated Protein A (SPA); anti-SPA antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against SPA. It has beenselected for its ability to recognize SPA in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-SPA antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Fragment
SPA (Asn21~Phe248)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Rat
Conjugate
No Conjugate
Immunogen
Recombinant SPA (Asn21~Phe248) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2049254
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SPA, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant SPA, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Lung Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Lung Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26,289 Da
NCBI Official Full Name
pulmonary surfactant-associated protein A isoform 1
NCBI Official Synonym Full Names
surfactant protein A1
NCBI Official Symbol
Sftpa1
NCBI Official Synonym Symbols
PSAP; SP-A; PSP-A; Sftp1; Sftpa; Sftpl
NCBI Protein Information
pulmonary surfactant-associated protein A
UniProt Protein Name
Pulmonary surfactant-associated protein A
Protein Family
UniProt Gene Name
Sftpa1
UniProt Synonym Gene Names
Sftp-1; Sftp1; Sftpa; PSAP; PSP-A; SP-A
NCBI Description
binds LPS and modifies LPS-induced immune cell activation in the lung [RGD, Feb 2006]
Uniprot Description
In presence of calcium ions, it binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. Enhances the expression of MYO18A/SP-R210 on alveolar macrophages.
Research Articles on SPA
Similar Products
Product Notes
The SPA sftpa1 (Catalog #AAA2028599) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Surfactant Associated Protein A (SPA) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Surfactant Associated Protein A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the SPA sftpa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Surfactant Associated Protein A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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